Wednesday, July 14, 2021

[Herpetology • 2021] First Records of the Fanged Frogs Limnonectes bannaensis and L. utara (Anura: Dicroglossidae) in Thailand

 (A, B) กบห้วยขาปุ่มน่าน Limnonectes bannaensis Ye, Fei & Jiang, 2007
กบห้วยขาปุ่มมลายู L. utara Matsui, Belabut & Ahmad, 2014 

in Suwannapoom, Jiang, Wu, ... et Che, 2021. 

Background: The taxonomic status of the Thai populations belonging to the Limnonectes kuhlii species complex is controversial, due to phenotypic similarity in the cryptic species complex. Recently, some studies on this group in Thailand have discovered four new species: L. taylori, L. megastomias, L. jarujini and L. isanensis. Even so, the diversity of this group is still incomplete.

New information: Based on an integrative approach encompassing genetic and morphological analyses, we conclude that the Limnonectes populations from Nan Province (northern) and Yala Province (southern) of Thailand are conspecific with L. bannaensis Ye, Fei & Jiang, 2007 and L. utara Matsui, Belabut & Ahmad, 2014, respectively. These are the first records of these species in Thailand. Our study highlights the importance of using DNA sequence data in combination with morphological data to accurately document species identity and diversity. This is especially important for morphologically cryptic species complexes and sympatrically occurring congeners.

Keywords: Nan Province, Yala Province, 16S rRNA, Cryptic species, Species complex

Figure 1. Map showing the type-localities of Limnonectes kuhlii species complex in Thailand. Circles = Type localities of L. taylori (red), L. jarujini (purple), L. megestomias (orange) and L. isanensis (brown). Blue square (L. bannaensis) and yellow square (L. utara) represent the two new distribution records in Thailand reported here.

Figure 2. BI tree resulting from 492 bp length fragment of mitochondrial 16S rRNA gene for Limnonectes species and outgroups. Bayesian posterior probabilities (BPP) > 95%/ML inferences (ML-BS) > 80% are shown for each node; “-” denotes low support of Bayesian posterior probabilities and bootstrap support < 80% in one analysis, no values on branches represent low support in both analyses. The scale bar represents 0.02 nucleotide substitutions per site.

Figure 4. Colour variations of Limnonectes bannaensis 
A. Dorsal view of male (AUP-00481); B. Dorsal view of female (AUP-00491).

Figure 5. Habitat of Limnonectes bannaensis in Bo Kluea District, Nan Province, northern Thailand.

Limnonectes bannaensis Ye, Fei, Xie & Jiang, 2007

Limnonectes utara Matsui, Belabut & Ahmad, 2014

Figure 6. Male of Limnonectes utara (AUP-01708) in life.
A. Dorsal view; B. Lateral view of head; C. Dorsolateral view of head; D. Dorsal view of leg (notice the tubercles); E. Ventral view of foot; F. Ventral view of hand.

Figure 7. Habitat of Limnonectes utara in Bannang Sata District, Yala Province, southern Thailand.

 Chatmongkon Suwannapoom, Ke Jiang, Yun-He Wu, Parinya Pawangkhanant, Sengvilay Lorphengsy, Tan Van Nguyen, Nikolay A. Poyarkov and Jing Che. 2021. First Records of the Fanged Frogs Limnonectes bannaensis Ye, Fei & Jiang, 2007 and L. utara Matsui, Belabut & Ahmad, 2014 (Amphibia: Anura: Dicroglossidae) in Thailand. Biodiversity Data Journal. 9: e67253. DOI: 10.3897/BDJ.9.e67253

กบห้วยขาปุ่มเหนือ, กบห้วยขาปุ่มเทย์เลอร์ Limnonectes taylori 
กบห้วยขาปุ่มใต้, กบห้วยขาปุ่มจารุจินต์ Limnonectes jarujini 
 กบห้วยปากใหญ่ Limnonectes megastomias 
กบห้วยขาปุ่มอีสาน, กบหมื่น, กบมื่น Limnonectes isanensis
กบห้วยขาปุ่มน่าน Limnonectes bannaensis 
 กบห้วยขาปุ่มมลายู Limnonectes utara