Abstract
During an amphibian biodiversity survey conducted in Tianlin County, Guangxi Zhuang Autonomous Region, China, a new species belonging to the genus Boulenophrys was identified and described as Boulenophrys tianlinensis sp. nov. This new species shares the closest genetic affinity with B. anlongensis (Li, Lu, Liu & Wang, 2020). Integrative taxonomic analyses, combining morphological characteristics and molecular phylogenetic data, confirm its status as a distinct species. The new species is distinguished from its congeners by a combination of the following characteristics: (1) medium body size, with snout-vent length (SVL) 34.3–39.0 mm in seven adult males and 47.1 mm in a single adult female specimen; (2) upper eyelid margin bearing one small horn-shaped tubercle on each side; (3) vomerine ridges present and vomerine teeth absent; (4) tongue not notched posterior; (5) tympanum rounded and clearly discernible; (6) toes with rudimentary webbing and lacking lateral fringes; (7) heels overlapping when thighs are held perpendicular to the body axis; (8) tibiotarsal articulation reaching the middle of the eye when the leg is stretched forwards; (9) males possessing a single subgular vocal sac; and (10) in breeding males, the nuptial pads have dark brown nuptial spines on the bases of the first and second fingers.
Key Words: Boulenophrys tianlinensis sp. nov., morphology, new species, phylogeny, taxonomy
Boulenophrys tianlinensis sp. nov.
Diagnosis.
Boulenophrys tianlinensis sp. nov. is assigned to the genus Boulenophrys, based on molecular phylogenetic analyses and the following generic diagnostic characters: head not enlarged and depressed; snout shield-like, projecting beyond the lower jaw; canthus rostralis distinct; vomerine ridges present and vomerine teeth absent; temporal region short; transverse fold behind head absent; both sides of belly without seriate tubercles; chest glands small and round, closer to the axilla than to mid-ventral line; femoral glands on rear part of thigh; vertical pupils; and the presence of subarticular tubercles at the base of each finger; nuptial spines are present on the dorsal bases of fingers I and II in breeding males (Fei et al. 2016; Lyu et al. 2023).
Boulenophrys tianlinensis sp. nov. can be distinguished from its congeners by a combination of the following morphological characters: (1) medium body size, with SVL 34.3–39.0 mm in seven adult males and 47.1 mm in a single adult female specimen; (2) upper eyelid margin bearing one small horn-shaped tubercle on each side; (3) vomerine ridges present and vomerine teeth absent; (4) tongue not notched posterior; (5) tympanum rounded and clearly discernible; (6) toes with rudimentary webbing and lacking lateral fringes; (7) heels overlapping when thighs are held perpendicular to the body axis; (8) tibiotarsal articulation reaching the middle of the eye when the leg stretched forwards; (9) males possessing a single subgular vocal sac; and (10) in breeding males, the nuptial pads have dark brown nuptial spines on the bases of the first and second fingers.
Jing Liu, Yanlin Cheng, Tuo Shen, Chaobo Feng, Lang Mu, Junjian Zhou, Gang Wei and Shize Li. 2026. A New Species of the Genus Boulenophrys (Anura, Megophryidae) from western Guangxi, China. Zoosystematics and Evolution. 102(5): 1139-1157. DOI: doi.org/10.3897/zse.102.193995 [15 Sep 2026]









































