[Herpetology • 2026] Geckolepis bekira, G. spania & G. modesta • Reverse Taxonomy of Fish-scale Geckos (Gekkonidae: Geckolepis) using shotgun sequencing for museomics-based assignment of Historical Names to Genetic Lineages

 

 species and subspecies of Geckolepis
(C) Geckolepis bekira sp. nov.; (B) G. spania sp. nov.

Vences, Glaw, Köhler, Miralles, Preez, Preick, Rakotoarison, Scherz, Hofreiter and Petzold, 2026

DOI: doi.org/10.11646/megataxa.21.2.1

Abstract

Fish-scale geckos (Geckolepis), endemic to Madagascar and the Comoro Islands, are nocturnal, arboreal lizards with large, imbricate scales that can be shed and regenerated in their entirety, most likely as an antipredator mechanism. Their species-level taxonomy has remained partly unresolved because morphology alone has proven insufficient to reliably assign genetic lineages to scientific names. Here, we use a museomics approach to extract and shotgun-sequence DNA from twelve liquid-preserved historical and mostly name-bearing type specimens of six historical nomina in the genus, and assemble sequences of the mitochondrial genes for 12S rRNA, 16S rRNA, and nd4 for ten of these specimens. Despite relatively low coverage, the assembled sequences robustly placed all these type specimens in a phylogenetic tree with 113 ingroup samples collected across Madagascar. Our results demonstrate the efficiency of this approach to recover mitochondrial evidence for taxonomic purposes, but also show its failure to recover reads matching single-copy nuclear genes. We discuss the promise of a renewed focus on nuclear ribosomal genes as complementary nuclear-encoded DNA barcodes for vertebrates, as we found 18S rRNA sequences to be well-represented among the reads of the historical specimens studied here. Based on evidence from the mitochondrial tree, two nuclear-encoded genes (C-MOS, RAG-1), and morphology, we resurrect G. typica var. modesta Methuen & Hewitt, 1913 as a full species, G. modesta, from north-western Madagascar, confirm the current use of the name G. maculata Peters, 1880 for specimens from northern and eastern Madagascar, of G. polylepis Boettger, 1893 for specimens from western and north-western Madagascar, and of G. typica Grandidier, 1867 for specimens from southern and south-western Madagascar. We also recognize G. typica var. anomala Mocquard, 1902 as a subspecies, G. typica anomala, and confirm G. petiti Angel, 1942 to be a synonym of this subspecies. We also describe two new species, G. bekira sp. nov. and G. spania sp. nov. from northern Madagascar. The new classification provides a robust baseline to identify the remaining taxonomic issues in the genus and tackle them, preferably using genomic approaches which are also promising to better understand the molecular mechanisms of scarless wound healing and skin regeneration in these geckos.

Squamata, Gekkonidae, Geckolepis spania sp. nov., Geckolepis bekira sp. nov., Madagascar, museomics, archival DNA, Pisces

Representative photos in life of all species and subspecies of Geckolepis according to the classification proposed herein. All specimens except G. modesta and G. maculata from Manombo correspond to genotyped individuals. Not to scale.

Photos of the preserved holotypes of the two new species described herein,
Geckolepis spania sp. nov. and Geckolepis bekira sp. nov.
 For each species, lateral, dorsal and ventral views of head, and dorsal view of entire specimen are shown.

Photos in life of the holotypes of the two new species described herein.
(A, B) Geckolepis spania sp. nov., holotype, probably a subadult, ZSM 2124/2007 (FGZC 1140) from Ankarana.
(C, D) Geckolepis bekira sp. nov., the adult male holotype ZSM 1523/2008 (FGZC 1971) from Montagne des Français.

Geckolepis bekira sp. nov.

Diagnosis. Assigned to the genus Geckolepis based on molecular phylogenetic relationships and body covered with large, imbricated scales. Based on morphological data of Lemme et al. (2013), characterized by snout–vent length <54 mm, 25–28 scales around midbody, 33–43 ventral scale rows, condition A or B of mental scale, eight supralabials, and usually a dark stripe from eye to ear opening. The new species differs from G. humbloti, G. maculata, G. megalepis and G. modesta by a smaller body size (SVL in these species reaching up to 65–75 mm), from G. megalepis and G. modesta furthermore by more scales around midbody (25–28 vs. 17–25), and from G. megalepis also by more ventral scale rows (33–43 vs. 27–32); from G. typica by a different condition of mental scale (A or B vs. D); from G. polylepis by having fewer ventral scale rows (33–43 vs. 48–55) and more scales around midbody (25–28 vs. 35–38). The new species can be differentiated from all other species and lineages in the genus complex by numerous diagnostic nucleotide positions in the mitochondrial genome. MolD identified for the nd4 fragment a robust diagnostic nucleotide combination of an “A” at site 904, a “C” at site 975, and a “T” at site 1002 (positions relative to the full nd4 gene of Geckolepis).

Etymology. The species name is derived from the Malagasy words be (= big) and kira (= scale), referring to the large imbricated scales that distinguish this and other species of Geckolepis from other Malagasy geckos. The species epithet is an invariable noun in apposition to the generic name.

Geckolepis spania sp. nov. 

Diagnosis. Assigned to the genus Geckolepis based on molecular phylogenetic relationships and body covered with large, imbricated scales. Characterized by small body size (but holotype is probably not adult), 26 scales around midbody, 34 ventral scale rows, condition A of mental scale, 7–8 supralabials, and presence of a dark stripe from eye to ear opening. The new species differs from G. modesta and G. megalepis by more scales around midbody (26 vs. 17–25), and from G. megalepis also by more ventral scale rows (34 vs. 27–32); from G. maculata (A+B) by more scales around midbody (26 vs. 22–25); from G. maculata (C) by fewer ventral scale rows (34 vs. 36–42); from G. humbloti by a larger number of supralabials compared to most specimens of that species (7–8 vs. 5–7); from G. typica by a different condition of mental scale (A vs. D); from G. polylepis by having fewer ventral scale rows (34 vs. 48–55) and fewer scales around midbody (26 vs. 35–38); reliable morphological differentiation from G. bekira is not possible at present. The new species can be differentiated from all other species and lineages in the genus complex by numerous diagnostic nucleotide positions in the mitochondrial genome. MolD identified for the nd4 fragment a robust diagnostic nucleotide combination of an “A” at site 876, and a “C” at site 943 (positions relative to the full nd4 gene of Geckolepis).

Etymology. The species name is a latinized adjective derived from Greek σπάνιος (spánios = rare), used in the feminine gender in agreement with the genus name. It refers to the rare occurrence of this species, which is known from a single collected specimen, despite numerous herpetological explorations of its type locality, Ankarana National Park. 

  

Miguel VENCES, Frank GLAW, Jörn KÖHLER, Aurélien MIRALLES, Louis Du PREEZ, Michaela PREICK, Andolalao RAKOTOARISON, Mark D. SCHERZ, Michael HOFREITER and Alice PETZOLD. 2026. Reverse Taxonomy of Fish-scale Geckos (Geckolepis) using shotgun sequencing for museomics-based assignment of Historical Names to Genetic Lineages. Megataxa. 21(2); 207-232. DOI: doi.org/10.11646/megataxa.21.2.1 [2026-09-22]

[Mammalogy • 2026] Phylogenomics and Museomics reveal Five distinct Species of Tiger Cats (Carnivora: Felidae: Leopardus) in South America

 

Leopardus tilcayo  Nogales-Ascarrunz, Aliaga-Rossel, Lescroart & Eizirik, 

in Lescroart, Nogales-Ascarrunz, Cassatt-Johnstone, Bonilla-Sánchez, ... et Eizirik, 2026. 

DOI: doi.org/10.1016/j.cub.2026.08.059

Highlights: 

• First discovery of a novel cat species in over a century: Leopardus tilcayo

• Description of a novel cat subspecies: Leopardus tigrinus antisuyo

• Phylogenomic resolution of the tiger cat complex, revealing five distinct species

• Museomics and novel approaches to assess evolutionary history and genomic diversity

Summary

The evolutionary history of elusive organisms can be characterized through genomic analyses, which have the power to reveal previously unknown taxa even in groups assumed to be well studied, such as cats. We have analyzed complete genomes of 38 individuals from the Neotropical cat genus Leopardus, including 26 individuals representing multiple evolutionary units of the contentious tiger cat (Leopardus tigrinus) species complex. Eight genomes were generated from museum specimens, which allowed the first genetic assessment of the type locality for L. tigrinus in the Guiana Shield. We found that this complex comprises five distinct species, including a novel cat species, discovered in the Bolivian Yungas and described in this study as L. tilcayo. The Peruvian Yungas unit of this complex also represents a distinct taxonomic entity, which we describe here as a novel subspecies, L. tigrinus antisuyo. Our phylogenomic analyses resolve the evolutionary relationships among the tiger cat geographic units, thus stabilizing their recalcitrant taxonomy and enabling adequate conservation assessment of these threatened felids. We also address other aspects of their evolution, including biogeography, past episodes of interspecies admixture, demographic history of each taxonomic unit, and temporal changes in genetic diversity. Altogether, our results clarify the evolutionary history of a complex radiation of wild cats, reveal novel taxa, and serve as a basis for conservation planning on behalf of these elusive wild cats.

 

Keywords: Bolivian Yungas, Peruvian Yungas, Felidae, Guiana Shield, historic DNA, Leopardus, museomics, concordance vector, new species, tilcayo

 

Live specimen of Leopardus tilcayo (sample ID: L. tilcayo BOl-2, voucher ID: MNHN-L2), a newly described tiger cat species from the Bolivian Yungas.

Photo by Paola Nogales-Ascarrunz

 

Family Felidae Batsch, 1788 

Subfamily Felinae Batsch, 1788 

 Leopardus Gray, 1842

Leopardus tilcayo sp. nov. Nogales-Ascarrunz, Aliaga-Rossel, Lescroart & Eizirik 

Type material: Holotype: CBF-11990, adult male. Live-captured and held in captivity, genome sequences NCBI SRA accession SRX30196846. Date: 09-2021; collectors: E. Aliaga-Rosel and P. Nogales-Ascarrunz (see Data S1).

Type locality: La Paz, Nor Yungas, near Arapata village (1,570 m above sea level [a.s.l.]), Bolivia. Coordinates: −16.248594, −67.634691 (WGS84).

Etymology: This species is named after the local name “tilcayo” used for this felid, which is widely known in the Bolivian Yungas zone. The name is treated as an unchanged noun in apposition.

Vernacular name: We propose “tilcayo” as the English common name, preserving the traditional local designation in recognition of its cultural and regional relevance.

Diagnosis: Leopardus tilcayo differs from L. pardinoides by its shorter pelage, lower contrast pattern, more expanded and irregular open rosettes, less bushy tail, and the absence of broad and complete caudal bands (see Data S1). It differs from L. tigrinus by its larger and more irregular flank markings, longer and fuller tail, and irregular caudal blotches. It differs from L. guttulus by its larger, more heterogeneous markings, lower dorsal contrast, and relatively longer tail. L. tilcayo can be distinguished from other Leopardus taxa by a large number of molecular characters. In the nuclear genome, among the 29,288,951 transversion variants, on average 1,392,646 sites differentiate L. tilcayo from L. t. antisuyo and 1,491,301 sites differentiate L. tilcayo from L. pardinoides. For simplicity we provide a list of diagnostic mitochondrial DNA sites (see Data S1).

Jonas Lescroart, Paola Nogales-Ascarrunz, Molly Cassatt-Johnstone, Alejandra Bonilla-Sánchez, Caroline Charão Sartor, Fernando Angulo, Jorge L. Ramirez, Larissa Rosa de Oliveira, Tadeu Gomes de Oliveira, Beth Shapiro, Enzo Aliaga-Rossel, Hannes Svardal and Eduardo Eizirik. 2026. Phylogenomics and Museomics reveal Five distinct Species of Tiger Cats in South America. Current Biology. DOI: doi.org/10.1016/j.cub.2026.08.059 [September 17, 2026]

[Herpetology • 2025] Dryadobates gen. nov., Dryadobates bokermanni, D. lutzi ... • Museomics and the Systematics of the Atlantic Forest Nurse Frogs (Dendrobatoidea: Aromobatidae: Allobatinae)

 

Dryadobates lutzi

Dryadobates bokermanni

Grant, Lyra, Hofreiter, Preick, Barlow, Verdade & Rodrigues, 2025

DOI: doi.org/10.1206/0003-0090.472.1.1 

 

Abstract

For four decades after 1967, four species of nurse frogs were recognized in the Atlantic Forest, namely Allobates alagoanus (Bokermann, 1967), A. capixaba (Bokermann, 1967), A. carioca (Bokermann, 1967), and A. olfersioides (A. Lutz, 1925), but in 2007 they were synonymized due to a lack of morphological differences. Although growing evidence from DNA and bioacoustics suggests that multiple species of nurse frogs inhabit the Atlantic Forest, their taxonomy has not been updated because populations at the four type localities had all vanished by the 1990s, making it impossible to collect tissues for DNA analysis and other data (e.g., vocalizations) from topotypic material. To overcome the lack of modern tissues, we employed museomics to obtain historical DNA from topotypic material of the four nominal species, which we analyzed together with data from extant populations from throughout the Atlantic Forest and Atlantic Forest enclaves within the Caatinga. We found that the Atlantic Forest nurse frogs comprise a well-supported clade of no fewer than 12 species that arrived in the Atlantic Forest via a single invasion from the Guiana Shield. We propose Dryadobates, gen. nov., for this clade, which is the sister group of all other allobatines. We consider the four nominal species to be valid, redescribe them as D. alagoanus, comb. nov., D. capixaba, comb. nov., D. carioca, comb. nov., and D. olfersioides, comb. nov., describe two sister species (D. bokermanni, sp. nov., and D. lutzi, sp. nov.) from southern Bahia, and summarize available information for the remaining six undescribed species. The type series of D. olfersioides comprises two species, so we designate a lectotype to clarify the application of the name. Dryadobates alagoanus is extant and broadly distributed, but D. capixaba, D. carioca, and D. olfersioides are presumed extinct, representing 50% of the nominal species of Dryadobates. These results provide a clear and consequential example of the essential role museomics and taxonomy play in understanding diversity loss and setting conservation priorities.

 

Keywords: Dendrobatidae, Mata Atlântica, Classification, Frogs, Genetics, Dryadobates, Museomics.

Dryadobates bokermanni, sp. nov., adult in life.
Adult male holotype (MZUSP 160849, 16.7 mm SVL): A, dorsal view; B, ventral view.
C, adult female topoparatype (MZUSP 160848, 17.8 mm SVL), ventral view. Photographs: T.G.

Dryadobates lutzi, sp. nov., adult male paratypes in life.
 A, dorsal view (MZUSP 160544, 14.7 mm SVL; photograph: M.T.R.);
B, ventral view (MZUESC 20074, 15.5 mm SVL; photograph: Omar Rojas Padilla).

Dryadobates gen. nov.

Dryadobates bokermanni sp. nov.

Dryadobates lutzi sp. nov.

Dryadobates olfersioides comb. nov.

Dryadobates alagoanus , D. capixaba , D. carioca (removed from the synonymy of Dryadobates olfersioides)

Dryadobates alagoanus adult male in life (an individual in the series CHUFPE 1817–1823):
A, dorsal view; B, ventral view.

Photographs: Marcos Dubeux.

 

Taran Grant, Mariana L. Lyra, Michael Hofreiter, Michaela Preick, Axel Barlow, Vanessa K. Verdade, Miguel Trefaut Rodrigues. 2025. Museomics and the Systematics of the Atlantic Forest Nurse Frogs (Dendrobatoidea: Aromobatidae: Allobatinae). Bulletin of the American Museum of Natural History. 2025(472); 1-76. DOI: doi.org/10.1206/0003-0090.472.1.1  [6 May 2025]

https://hdl.handle.net/2246/7504

[Ornithology • 2026] Pulsatrix perspicillata & Pulsatrix pulsatrix • Museomics resolves 200 years of Taxonomic Uncertainty: Strix pulsatrix Wied, 1820 (Strigiformes: Strigidae) is A Valid Species

in Bolívar-Leguizamón, Corrêa, Bencke et Silveira. 2026.

DOI: doi.org/10.1016/j.ympev.2025.108488

Artwork by Eduardo Brettas
 instagram.com/EduardoBrettas

 

Highlights

• Pulsatrix perspicillata is an owl found from Mexico to Argentina and Brazil.

• The taxonomic position of the P. p. pulsatrix subspecies is a matter of controversy.

• Historical DNA shows great divergence between the pulsatrix and perspicillata taxa.

• The pulsatrix-perspicillata clade is sister to a melanota-koeniswaldiana clade.

• Pleistocene climatic oscillations influenced the diversification of the genus.

• We proposed elevating the subspecies pulsatrix of P. perspicillata to species status.

Abstract

The identification of valid species is central to biology, and genetic data have been essential in uncovering new taxonomic units across groups. For polytypic taxa, genetics helps distinguish valid units from natural variation. Pulsatrix perspicillata (Spectacled Owl) is a widespread polytypic species with six recognized subspecies. We used genetic data and museomics techniques to: (i) test the validity of Strix pulsatrix Wied, 1820 (=Pulsatrix perspicillata pulsatrix) as a species distinct from Pulsatrix perspicillata; and (ii) estimate the phylogenetic relationships and divergence times within the genus Pulsatrix Kaup, 1848. We conducted population structure and phylogenetic analyses using SNPs matrices and alignments of Ultraconserved Elements (UCEs) from 16 individuals of Pulsatrix spp., including four historical samples of P. p. pulsatrix. Additionally, we sequenced the mtDNA-ND2 gene from 38 samples representing all known Pulsatrix species to further elucidate phylogenetic relationships and estimate divergence times. Our results indicate that Pulsatrix perspicillata pulsatrix represents an independent lineage from P. perspicillata, as supported by its reciprocal monophyly and distinct population structure. UCE analyses also grouped P. koeniswaldiana (Tawny-browed Owl) and P. melanota (Band-bellied Owl) into a distinct cluster. Phylogenetic analyses based on both UCEs and mtDNA-ND2 sequences support the monophyly of the genus Pulsatrix. Pulsatrix koeniswaldiana and P. melanota form a monophyletic group that is sister to P. perspicillata. Pulsatrix diverged in the Late Miocene and diversified in the Pliocene-Quaternary. The evolutionary history of Pulsatrix appears to have been influenced by (a) the final phase of the Andean uplift and (b) climatic oscillations during the Pleistocene. Pulsatrix p. pulsatrix shows genetic divergence consistent with a species-level split from P. perspicillata, and we henceforth recognize it as Pulsatrix pulsatrix (Wied 1820). We recommend future studies to assess its current distribution and inform the development of conservation strategies.

 

Keywords: Museomics, UCEsmt-ND2, Pulsatrix, Spectacled Owl, Neotropics, Systematics, Phylogeography 

 

Pulsatrix perspicillata 

Sergio D. Bolívar-Leguizamón, Aline Henrique Corrêa, Glayson Ariel Bencke and Luís F. Silveira. 2026. Museomics resolves 200 years of Taxonomic Uncertainty: Strix pulsatrix Wied, 1820 (Strigiformes, Strigidae) is A Valid Species. Molecular Phylogenetics and Evolution. 108488. DOI: doi.org/10.1016/j.ympev.2025.108488 [27 October 2025]

 instagram.com/p/DSI6x84EpMR