[Herpetology • 2026] Boulenophrys tianlinensis • A New Species of the Genus Boulenophrys (Anura: Megophryidae) from western Guangxi, China

Boulenophrys tianlinensis 

Liu, Cheng, Shen, Feng, Mu, Zhou, Wei & Li, 2026

 

DOI: doi.org/10.3897/zse.102.193995

Abstract

During an amphibian biodiversity survey conducted in Tianlin County, Guangxi Zhuang Autonomous Region, China, a new species belonging to the genus Boulenophrys was identified and described as Boulenophrys tianlinensis sp. nov. This new species shares the closest genetic affinity with B. anlongensis (Li, Lu, Liu & Wang, 2020). Integrative taxonomic analyses, combining morphological characteristics and molecular phylogenetic data, confirm its status as a distinct species. The new species is distinguished from its congeners by a combination of the following characteristics: (1) medium body size, with snout-vent length (SVL) 34.3–39.0 mm in seven adult males and 47.1 mm in a single adult female specimen; (2) upper eyelid margin bearing one small horn-shaped tubercle on each side; (3) vomerine ridges present and vomerine teeth absent; (4) tongue not notched posterior; (5) tympanum rounded and clearly discernible; (6) toes with rudimentary webbing and lacking lateral fringes; (7) heels overlapping when thighs are held perpendicular to the body axis; (8) tibiotarsal articulation reaching the middle of the eye when the leg is stretched forwards; (9) males possessing a single subgular vocal sac; and (10) in breeding males, the nuptial pads have dark brown nuptial spines on the bases of the first and second fingers.

Key Words: Boulenophrys tianlinensis sp. nov., morphology, new species, phylogeny, taxonomy

Male holotype MT TL20230603004 of Boulenophrys tianlinensis sp. nov. in life.
A. Dorsal view; B. Ventral view; C. Dorsal view of hand; D. Ventral view of hand; E. Ventral view of foot.
Male holotype CIB AL20190531018 of B. anlongensis in life; F. Dorsal view; G. Ventral view; H. Dorsal view of hand; I. Ventral view of hand; J. Ventral view of foot.

photos by Shize Li.

Colour variation in Boulenophrys tianlinensis sp. nov. in life: 

Dorsolateral and ventral view of adult;

[A] male MT TL20230603001; [B] male MT TL20230603003;
[C] male MT TL20230603005; [D] female MT TL20230603002.

photos by Shize Li.

Boulenophrys tianlinensis sp. nov.

Diagnosis.

Boulenophrys tianlinensis sp. nov. is assigned to the genus Boulenophrys, based on molecular phylogenetic analyses and the following generic diagnostic characters: head not enlarged and depressed; snout shield-like, projecting beyond the lower jaw; canthus rostralis distinct; vomerine ridges present and vomerine teeth absent; temporal region short; transverse fold behind head absent; both sides of belly without seriate tubercles; chest glands small and round, closer to the axilla than to mid-ventral line; femoral glands on rear part of thigh; vertical pupils; and the presence of subarticular tubercles at the base of each finger; nuptial spines are present on the dorsal bases of fingers I and II in breeding males (Fei et al. 2016; Lyu et al. 2023).

Boulenophrys tianlinensis sp. nov. can be distinguished from its congeners by a combination of the following morphological characters: (1) medium body size, with SVL 34.3–39.0 mm in seven adult males and 47.1 mm in a single adult female specimen; (2) upper eyelid margin bearing one small horn-shaped tubercle on each side; (3) vomerine ridges present and vomerine teeth absent; (4) tongue not notched posterior; (5) tympanum rounded and clearly discernible; (6) toes with rudimentary webbing and lacking lateral fringes; (7) heels overlapping when thighs are held perpendicular to the body axis; (8) tibiotarsal articulation reaching the middle of the eye when the leg stretched forwards; (9) males possessing a single subgular vocal sac; and (10) in breeding males, the nuptial pads have dark brown nuptial spines on the bases of the first and second fingers.

 Jing Liu, Yanlin Cheng, Tuo Shen, Chaobo Feng, Lang Mu, Junjian Zhou, Gang Wei and Shize Li. 2026. A New Species of the Genus Boulenophrys (Anura, Megophryidae) from western Guangxi, China. Zoosystematics and Evolution. 102(5): 1139-1157. DOI: doi.org/10.3897/zse.102.193995 [15 Sep 2026]

[Herpetology • 2020] Boulenophrys anlongensis • A New Species of Boulenophrys (Anura: Megophryidae) from Guizhou Province, China

 Boulenophrys anlongensis (Li, Lu, Liu & Wang, 2020)

Megophrys anlongensis Li, Lu, Liu & Wang, 2020

安龙角蟾  | DOI: doi.org/10.3897/zookeys.986.57119

Abstract

A new species of the genus Megophrys is described from Guizhou Province, China. Molecular phylogenetic analyses based on mitochondrial DNA indicated the new species as a clade clustered into the Megophrys clade. The new species can be distinguished from its congeners by a combination of the following characters: body size moderate (SVL 40.0–45.5 mm in males and 48.9–51.2 mm in females); vomerine teeth absent; tongue not notched behind; tympanum distinctly visible, oval; a small horn-like tubercle at the edge of each upper eyelid; two metacarpal tubercles in hand; toes with rudimentary webbing; heels overlapping when thighs are positioned at right angles to the body; tibiotarsal articulation reaching the level of mid-eye when leg stretched forward; in breeding males, an internal single subgular vocal sac present and brownish nuptial pads, made up of black nuptial spines, present on the dorsal base of the first two fingers.

Keywords: Molecular phylogenetic analysis, morphology, new species, taxonomy

Photographs of the holotype CIBAL20190531018 of Megophrys anlongensis sp. nov. in life
A dorsal view B ventral view C dorsal view of hand D ventral view of hand. E ventral view of foot.

Colour variation in Megophrys anlongensis sp. nov. 
  male CIBAL20190531017: A dorsolateral view, B ventral view; 
 female CIBAL20190531022: C dorsolateral view, D ventral view;  
female CIBAL20190811014: E dorsolateral view, F ventral view. 

Megophrys anlongensis sp. nov.

  

Diagnosis: 

Megophrys anlongensis sp. nov. is assigned to the genus Megophrys based on molecular phylogenetic analyses and the following generic diagnostic characters: snout shield-like, projecting beyond the lower jaw; canthus rostralis distinct; chest glands small and round, closer to the axilla than to midventral line; femoral glands on rear part of thigh; vertical pupils.

Megophrys anlongensis sp. nov. could be distinguished from its congeners by a combination of the following morphological characters: (1) body size moderate (SVL 40.0–45.5 mm in males and 48.9–51.2 mm in females); (2) vomerine teeth absent; (3) tongue not notched behind; (4) a small horn-like tubercle at the edge of each upper eyelid; (5) tympanum distinctly visible, oval; (6) two metacarpal tubercles on hand; (7) toes with rudimentary ...

Etymology: The specific name anlongensis refers to the known distribution of this species, Anlong County, Guizhou Province, China. We propose the common English name “Anlong horned toad”, and Chinese name “An Long Jiao Chan” (安龙角蟾).

 Shi-Ze Li, Ning-Ning Lu, Jing Liu and Bin Wang. 2020. Description of A New Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys. 986: 101-126. DOI: doi.org/10.3897/zookeys.986.57119 [Nov 2020]

[Herpetology • 2026] Ingerana aizawl • Leaping from a University Campus to the Scientific Records: A New Species of Ingerana Dubois, 1987 (Anura: Dicroglossidae) from Mizoram, India

Ingerana aizawl

Lalremsanga, Bharali, Dawngliana, Hlychho, Malsawmtluanga, Bhattacharjee, Hazarika, Das & Purkayastha, 2026

 

DOI: doi.org/10.11646/zootaxa.5878.2.3

 

Abstract

Populations of Ingerana Dubois (Anura: Dicroglossidae) from Mizoram, northeastern India, have historically been assigned to Ingerana borealis (Annandale), however recent taxonomic reassessment questioned their identity. We evaluated these populations using an integrative approach combining external morphology and mitochondrial 16S rRNA sequence data. Phylogenetic analyses recovered the Mizoram population as a distinct lineage within Ingerana, while morphological comparisons revealed multiple diagnostic differences from recognised congeners. Based on these data, we recognise the Mizoram population as a distinct species. It differs from I. borealis, I. occidens, and I. tenasserimensis by a unique combination of body size, tympanic morphology, dorsal skin texture, and proportional morphometric characters. Our findings resolve the taxonomic identity of Ingerana populations from Mizoram and suggest that species diversity within the genus in northeastern India remains underestimated.

Amphibia, 16S rRNA, Aizawl, new species, northeastern India

Ingerana aizawl

HMAR TLAWMTE LALREMSANGA, MANMATH BHARALI, MALSAWM DAWNGLIANA, MARA DUASA HLYCHHO, MALSAWMTLUANGA, RUPANKAR BHATTACHARJEE, ARUP KUMAR HAZARIKA, NILUTPAL DAS and JAYADITYA PURKAYASTHA. 2026. Leaping from a University Campus to the Scientific Records: A New Species of Ingerana Dubois, 1987 (Anura: Dicroglossidae) from Mizoram, India.  Zootaxa. 5878(2); 224-236. DOI: doi.org/10.11646/zootaxa.5878.2.3 [2026-09-14]

[Herpetology • 2026] Duttaphrynus phawngpui • A New Montane Toad Species (Anura: Bufonidae: Duttaphrynus) from Mizoram, northeastern India

Duttaphrynus phawngpui 

 Dawngliana, Warjri, Amarasinghe, Bal, Hruaia, Hlychho, Lalremsanga, Vabeiryureilai & Purkayastha, 2026

DOI: doi.org/10.47605/tapro.v15i2.423  

 Researchgate.net/publication/414271782

Abstract

We describe a new montane bufonid toad from Phawngpui National Park, Mizoram, northeastern India, using an integrative taxonomic approach combining morphology, multivariate morphometrics, and mitochondrial 16S rRNA sequence data. Phylogenetic analyses recover the Mizoram population as a well-supported lineage within the Duttaphrynus stuarti species group, distinct from its closest congeners, including D. stuarti, D. chandai, and D. dhara. Uncorrected 16S sequence divergence from currently recognised taxa ranges from 3.8–5.5%, consistent with species-level differentiation in Asian bufonids. Principal component analysis of size-corrected morphometric variables further supports its distinctiveness, with the new species occupying a discrete region of morphospace. The new species is characterized by moderate body size (SVL 47.72–56.26 mm), indistinct tympanum, absence of most cranial ridges, distinctive digital proportions, moderate toe webbing, and a dorsum with glandular warts, irregular dark blotches, and a narrow mid-dorsal stripe. Concordant molecular, morphometric, and morphological evidence supports its recognition as a distinct species. Known only from the type locality in high-elevation (>2000 m a.s.l.) montane habitats, the species highlights the importance of the Mizoram uplands for undocumented amphibian diversity within the Indo-Burma biodiversity hotspot.

Duttaphrynus phawngpui sp. nov 

Malsawm Dawngliana, Holiness Warjri, A. A. Thasun Amarasinghe, Amit K. Bal, Vanlal Hruaia, Mara D. Hlychho, Hmar T. Lalremsanga, Mathipi Vabeiryureilai and Jayaditya Purkayastha. 2026. A New Montane Toad Species (ANURA: BUFONIDAE: Duttaphrynus) from Mizoram, northeastern INDIA. TAPROBANICA. 15(2); 298–309. DOI: doi.org/10.47605/tapro.v15i2.423 [13 September 2026]  

 Researchgate.net/publication/414271782_A_NEW_MONTANE_TOAD_Duttaphrynus_from_MIZORAM_NE_INDIA

[Herpetology • 2026] Oreolalax lizipingensis • A New Oreolalax (Anura: Megophryidae) from southwest Sichuan, China

 

Oreolalax lizipingensis

Huang, Dai, Hou, Lin, Wang, Yu, Lin & Xie, 2026

栗子坪齿蟾  |  DOI: doi.org/10.3897/zse.102.183210

Abstract

The genus Oreolalax of the family Megophryidae currently comprises 22 recorded species, the majority of which are endemic to China. During field work in Liziping National Natural Reserve, Shimian County, Sichuan Province, China, we discovered several Oreolalax toads with morphological characteristics different from other Oreolalax species. Based on the analyses of morphological, bioacoustic and molecular data, this newly identified population forms an independent clade nested with Oreolalax lichuanensis, Oreolalax schmidti and Oreolalax pingii, which we describe as a new species. The new species, Oreolalax lizipingensis sp. nov., can be distinguished from other congeneric species by the combination of the following characters: (1) relatively small body size in Oreolalax, SVL of males 37.6–43.3 mm and females 44.2–52.7 mm; (2) toes with rudimentary webbing; (3) interorbital region with dark triangular spot; (4) the female’s belly, flanks and lower surface of limbs evenly dotted with white spots, absent or inconspicuous in males; (5) dorsum with dark brown and nearly round warts; (6) male with spiny warts along edge of jaw, ventral thighs and abdomen near vent; and (7) breeding season occurring from July to August. Oreolalax lizipingensis sp. nov. is currently known only from high-elevation streams at 2400–3100 m a.s.l. within the Menghuo Castle where it occurs in sympatry with Oreolalax liangbeiensis.

Key Words: New species, Oreolalax lizipingensis sp. nov., taxonomy

Photos of the holotype CIB22565 of male Oreolalax lizipingensis sp. nov. in life
 A. Dorsal view; B. Ventral view; C. Lateral view; D. Ventral view of hand and; E. Ventral view of foot. The scale bars for panels A, B, and C are uniform and located in the upper right corner.

Oreolalax lizipingensis sp. nov.

Diagnosis.

Oreolalax lizipingensis sp. nov. assigned to the genus Oreolalax based on its phylogenetic position and the following characters: pupil vertical; vocal sac absent; dorsal surface exhibiting dark-colored and nearly round tubercles; males possessing a pair of chest glands densely covered with black spines (Fei et al. 2009).

The new species differs from its congeners by a combination of the following morphological characters: (1) the body size relatively small, SVL of adult males 40.2 mm (37.6–43.3 mm, n = 5); adult females 47.7 mm (44.2–52.7 mm, n = 3); (2) head length slightly longer than head width; (3) tongue deeply notched posteriorly; (4) supratympanic fold narrow and elongated; (5) tympanum hidden; (6) hand without subarticular and ...

Etymology. The specific epithet “liziping” of the new species refers to the type locality of the species, Liziping National Nature Reserve, Sichuan Province, China. 

We suggest the common name as “Liziping toothed toad”, and the Chinese name as “Li Zi Ping Chi Chan (栗子坪齿蟾)”.

The amplexus, egg mass, and breeding habitat of Oreolalax lizipingensis sp. nov.
A. Amplexus in July; B. Pile-shaped gelatinous egg mass; C, D. Breeding habitat.

 Si Ning Huang, Qin Long Dai, Yin Meng Hou, Yi Heng Lin, Yuan Fei Wang, Hao Qi Yu, Xiu Qin Lin and Feng Xie. 2026. A New Oreolalax (Anura, Megophryidae) from southwest Sichuan, China. Zoosystematics and Evolution. 102(5): 1107-1125. DOI: doi.org/10.3897/zse.102.183210 [2 Sep 2026]

[Herpetology • 2026] Leptobrachella jinzhongshanensis • A New Species of Leptobrachella (Anura: Megophryidae) from Jinzhongshan National Nature Reserve, Guangxi, China

Leptobrachella jinzhongshanensis

Chen, Su, Yang & Pan, 2026

金钟山掌突蟾 | DOI: doi.org/10.3897/zse.102.195575 

Abstract

Integrative morphological and molecular evidence supports the recognition of Leptobrachella jinzhongshanensis sp. nov. from Longlin County, Guangxi, China. The new species is distinguished by a combination of characters, including medium body size (snout-vent length 30.4–32.5 mm in males and 40.3–42.8 mm in females), narrow lateral fringes on the toes, absence of toe webbing, continuous dermal ridges beneath the toes, heels that do not meet when the thighs are held at right angles to the body axis, irregular black flank spots, a bicoloured iris and interwoven creamy-white and light-grey markings on the venter. Phylogenetic analyses of mitochondrial 16S rRNA sequences recovered the Jinzhongshan specimens as a strongly supported lineage sister to L. dorsospina, from which they differed by an uncorrected p-distance of 1.4%. Leptobrachella jinzhongshanensis sp. nov. is currently known only from montane evergreen broadleaf forest at 1,700–1,800 m elevation. Within the Reserve, L. ventripunctata was recorded along streams below 1,000 m, indicating ecological and elevational separation between the two species.

Key Words: Integrative taxonomy, leaf-litter frog, morphology, phylogeny

Leptobrachella jinzhongshanensis sp. nov. 
 A–F. Holotype (NNU 001478): A. Dorsal view; B. Ventral view; C, D. Dorsolateral views; E. Ventral view of hand; F. Ventral view of foot.
G, H. Paratype NNU 001479: G. Dorsal view; H. Ventral view.

photographs by Wei-Cai Chen.

Variation in Leptobrachella jinzhongshanensis sp. nov.
 A1, A2. NNU 001323; B1, B2. NNU 001324;
C1, C2. NNU 001480; D1, D2. NNU 001481;
E1, E2. NNU 001482; F1, F2. NNU 001483;
G1, G2 NNU 001484; H1, H2 NNU 001485.

photographs by Wei-Cai Chen.

Leptobrachella jinzhongshanensis sp. nov.

Etymology. The specific epithet jinzhongshanensis refers to the type locality, Mt Jinzhongshan. We propose “Jinzhongshan Leaf-litter Frog” as the English common name and Jīn Zhōng Shān Zhǎng Tū Chán (金钟山掌突蟾) as the Chinese common name.

Diagnosis. Leptobrachella jinzhongshanensis sp. nov. is distinguished from congeners by the following combination of characters: (1) medium body size (SVL 30.4–32.5 mm in males and 40.3–42.8 mm in females); (2) narrow lateral fringes on the toes; (3) absence of toe webbing; (4) continuous dermal ridges beneath the toes; (5) relative finger lengths IV < II ≈ I < III; (6) tibiotarsal articulation reaching the posterior corner of the eye; (7) heels not meeting when the thighs are held at right angles to the body axis; (8) irregular black spots on the flanks; (9) iris copper above and silver below; and (10) interwoven creamy-white and light-grey markings on the venter.

 Wei-Cai Chen, Tong-Ping Su, Hai-Ju Yang and Mei-Ye Pan. 2026. A New Species of Leptobrachella (Anura, Megophryidae) from Jinzhongshan National Nature Reserve, Guangxi, China. Zoosystematics and Evolution. 102(4): 1089-1105. DOI: doi.org/10.3897/zse.102.195575  [27 Aug 2026]

[Herpetology • 2026] Amolops ammachi, A. bella & A. sendenyu • Systematics, Molecular Phylogeny, Diversification, and Biogeography of Cascade Frogs (Anura: Ranidae: Amolops) from India: An Integrative Assessment with Description of Three New Species

 

 the Amolops marmoratus group from India

A, B. Amolops afghanus. C. Amolops ammachi sp. nov.. D–F. A. assamensis. G–I. Amolops bella sp. nov.. J–M. A. gerbillus. N, O. A. indoburmanensis; 

A. Amolops indoburmanensis haplo 1. 

C. A. cf. indoburmanensis sp. 1.  E. A. jaunsari. 

F, A. nepalicus. H. Amolops sendenyu sp. nov. 

Biju, Ellepola, Sarmah, Upadhyaya, Bisht, Tajo, Hanken & Garg, 2026

DOI: doi.org/10.3099/MCZ88.1

Photos: S. D. Biju.

 Abstract 

The Cascade Frog genus Amolops, currently comprising 95 described species, is a taxonomically challenging group of Asian frogs. Although the genus has been studied extensively across most of its range, the Indian members remain poorly understood, with sporadic species descriptions and no comprehensive taxonomic revision to date. Additionally, the current taxonomy of Amolops in India remains in flux; the true species diversity is potentially underestimated due to insufficient sampling efforts, misidentifications arising from limited investigations, and lack of a comprehensive comparative framework. In this study, we addressed these gaps through extensive sampling of Amolops across their range in India and by using an integrative morphological and molecular approach to investigate existing diversity. By using a multilocus data set of mitochondrial (16S, CO1, ND2) and nuclear (RAG1, TYR) markers, we present the first comprehensive Amolops phylogeny, including all known Indian species, which enables us to confidently establish species boundaries and infer evolutionary relationships. By assigning available names to well-supported lineages and testing multiple species-delimitation hypotheses, we identified a high level of undescribed cryptic diversity in the genus. Integrative analyses confirm three distinct unnamed lineages, which are formally described here as Amolops ammachi sp. nov., Amolops bella sp. nov., and Amolops sendenyu sp. nov. In addition, we report five new country records for India: three genetically conspecific with Amolops afghanus, Amolops nepalicus, and Amolops wangyali, and two shallowly divergent populations of A. medogensis and A. bellulus. Conversely, five nominal species do not represent distinct taxa and are here treated as junior subjective synonyms: Amolops chanakya synonym of Amolops wangyali; Amolops mahabharatensis synonym of Amolops jaunsari; Amolops senchalensis synonym of Amolops formosus; Amolops shillong synonym of Amolops assamensis; and Amolops terraorchis synonym of Amolops gerbillus. Molecular species delimitation reveals six additional candidate species from India; these lineages are left unnamed due to shallow divergence from established species and warrant further taxonomic investigation. Our time-calibrated phylogenetic analyses suggest that Amolops diversification began around the Eocene-Oligocene boundary, with a mean age of 31.5 Mya (median age: 32.5 Mya; 95% highest posterior density: 28.12–36.67 Mya). The most recent common ancestor (MRCA) of extant Amolops was widely distributed across Southeast China, mainland Southeast Asia, and the Malayan Peninsula during the early Oligocene. Through subsequent range contraction, subdivision, and jump dispersal, they spread into adjacent regions with dramatic rapid diversification toward the late Miocene. Biogeographic analyses suggest multiple waves of east-to-west colonization and highlight the role of early Himalayan uplift in mediating a complex “into-and-out-of-Himalaya” dynamic in structuring the contemporary distribution and high cryptic diversity in the genus. Mitochondrial haplotypes also show strong geographic structuring, indicating historical allopatric divergence by secondary contact among regions. These findings additionally improve our broader understanding of diversification and distribution patterns in this unique group.

KEYWORDS: Amphibia, Anura, cryptic diversity, evolutionary history, Integrative taxonomy, Himalayas, South and Southeast Asia, species delimitation, monticola Group, viridimaculatus Group, marmoratus Group, tadpole morphology

 

Dorsolateral views of species within the Amolops marmoratus group from India, in life.
A, B. A. afghanus (SDBDU 2023.6459 and SDBDU 2021.322, respectively). C. Amolops ammachi sp. nov. (ZSI A 16551). D–F. A. assamensis (SDBDU 2022.1100, SDBDU 2018.3843, and SDBDU 2025.6858, respectively). G–I. Amolops bella sp. nov. (SDBDU 2011.6959, SDBDU 2011.6913, and SDBDU 2011.6956, respectively). J–M. A. gerbillus (SDBDU 2024.6597, SDBDU 2024.6726, SDBDU 2021.1225, and SDBDU 2024.6598, respectively). N, O. A. indoburmanensis (SDBDU 2018.3870 and SDBDU 2022.5572, respectively). Photos: S. D. Biju.

Amolops ammachi sp. nov.

Etymology. The species is named in honor of the late Ms. Padmini Varkey, a renowned social worker and philanthropist from Kerala, India. She is fondly referred to as “Ammachi,” meaning “mother” in Malayalam, the language of Kerala. Her unconditional love and readiness to help those in need were widely admired. The species epithet ammachi is a tribute to her life and spirit; it is treated as an invariable noun in apposition to the generic name.

Suggested Common Name. Ammachi's Cascade Frog.

Amolops bella sp. nov.

Etymology. The specific epithet bella is from the Latin word bellus, meaning beautiful, and referring to the beauty of the frog. The species epithet is considered an invariable noun in apposition.

Suggested Common Name. Beautiful Cascade Frog.

Dorsolateral views of species within the Amolops marmoratus group from India, in life.
 A. A. indoburmanensis haplo 1 (SDBDU 2024.6670). B. A. indoburmanensis haplo 2, (SDBDU 2021.4629). C. A. cf. indoburmanensis sp. 1 (SDBDU 2009.140). D, E. A. jaunsari (SDBDU 2012.6991 and SDBDU 2012.153, respectively). F, G. A. nepalicus (SDBDU 2026.6929 and SDBDU 2026.6928, respectively). H. Amolops sendenyu sp. nov. (SDBDU 2024.5438). I–K. A. siju (SDBDU 2023.457, SDBDU 2022.1468, and SDBDU 2025.6832, respectively). L. A. yarlungzangbo (SDBDU 2022.6141). Photos: S. D. Biju.

Amolops sendenyu sp. nov.

Etymology. The species is named after Sendenyu Village, Nagaland State, where the type series was collected. The species epithet is considered an invariable noun in apposition.

Suggested Common Name. Sendenyu Cascade Frog.

S. D. Biju, Gajaba Ellepola, A. N. Dikshit Akalabya Sarmah, Radhakrishna Upadhyaya, Karan Bisht, Tage Tajo, James Hanken and Sonali Garg. 2026. Systematics, Molecular Phylogeny, Diversification, and Biogeography of Cascade Frogs (Ranidae: Amolops) from India: An Integrative Assessment with Description of Three New Species. Bulletin of the Museum of Comparative Zoology. 164(1); 1-144. DOI: doi.org/10.3099/MCZ88.1 (11 August 2026)

 

[Herpetology • 2026] Boulenophrys calciphila • A New Species of Boulenophrys Fei, Ye & Jiang, 2016 (Anura: Megophryidae) from Karst Landscapes in northern Guangdong, China

 

Boulenophrys calciphila Zeng, Wang, Qi, Song, Lyu & Wang,

in Zeng, J. Wang, Qi, Song, Cai, Zhu, Lin, Li, Zhou, H.-C. Yang, Lyu, Y.-P. Yang et Y.-Y. Wang. 2026. 

DOI: doi.org/10.3897/herpetozoa.39.e202590

Abstract

Karst landscapes across southern China represent global hotspots for vertebrate diversity and endemism, yet amphibian diversity within such habitats remains insufficiently documented. Herein, we describe Boulenophrys calciphila sp. nov., a new species discovered from fragmented karst hills in northern Guangdong Province, China, extending the documented habitat spectrum of the genus in Guangdong to limestone karst terrain. Detailed morphological comparisons coupled with phylogenetic analyses validate its taxonomic distinctness from all known congeners. This new discovery aligns with ongoing records of range-restricted anuran species uncovered from southern Chinese karst formations, reinforcing karst ecosystems as important reservoirs of amphibian diversity. Our results demonstrate that amphibian diversity within poorly-explored karst regions of northern Guangdong is substantially underestimated and call for focused conservation actions to safeguard these microendemic taxa and their unique evolutionary lineages.

Key Words: Boulenophrys calciphila sp. nov., conservation priority, karst habitat, micro-endemism, taxonomy

Life aspect of Boulenophrys calciphila sp. nov.
 the male holotype (GEP a596): lateral view (A1), dorsal view (A2), ventral view (A3), ventral view of hand (A4), ventral view of foot (A5);
and the female paratype (GEP a594): lateral view (B1), dorsal view (B2), ventral view (B3), ventral view of hand (B4), ventral view of foot (B5).

Photo by Zhao-Chi Zeng.

Boulenophrys calciphila Zeng, Wang, Qi, Song, Lyu & Wang, sp. nov.

 

Common name. Limestone Horned Toad (in English) 

 huī yán jiǎo chán (灰岩角蟾 in Chinese).

Diagnosis. (1) Moderate size (SVL 49.7–52.7 mm in two adult males, 56.3–59.2 mm in five adult females); (2) snout rounded in dorsal view, canthus rostralis well developed, tongue not notched distally; (3) tympanum distinct; (4) vomerine ridges present, vomerine teeth absent; (5) dorsal skin smooth with sparse granules, discontinuous X-shaped ridge on centre of dorsum, discontinuous dorsolateral ridges present, sparse large tubercles on flanks, dorsal limbs with discontinuous transverse ridges and tubercles; (6) outer margin of upper eyelid with a prominent horn-like ...

Etymology. The specific epithet calciphila combines the Latin calx, genitive calcis (limestone) with the Greek φίλος (phílos) (= loving), in reference to the association of this species with limestone karst habitats.

 Zhao-Chi Zeng, Jian Wang, Shuo Qi, Han-Ming Song, Yao-Yu Cai, Yun-Sheng Zhu, Shi-Shi Lin, Yuan-Hang Li, Dan-Yang Zhou, Hao-Cong Yang, Zhi-Tong Lyu, Yi-Ping Yang and Ying-Yong Wang. 2026. A New Species of Boulenophrys Fei, Ye & Jiang, 2016 (Anura, Megophryidae) from Karst Landscapes in northern Guangdong, China. Herpetozoa. 39: 291-302. DOI: doi.org/10.3897/herpetozoa.39.e202590 [12-08-2026]

[Herpetology • 2026] Niceforonia tempestuosa • A New Species of Niceforonia (Anura: Strabomantidae) from the Serranía de los Paraguas, Colombia, with the Description of A New phenotypic synapomorphy for the Niceforonia nigrovittata species group

[A-D] Niceforonia tempestuosa 

Ospina-Sarria, Rodríguez-Betancourt, Gómez-Sepúlveda, Morales-Vertel, Vélez-Franco, Gallego-Posada, Herrera-Rengifo, Grajales Escobar, Ceballos-Castro, García-Gómez, Velásquez-Trujillo, Ortiz Baez & Mendez Narvaez, 2026

and [F, H] N. mantipus (Boulenger, 1908)

DOI: doi.org/10.3897/zookeys.1289.198411

Abstract

A new species of Niceforonia is described from the montane cloud forests of southwestern Colombia. The new species is distinguished from all congeners by possessing dorsolateral folds, a visible tympanic membrane, a fleshy lip swelling in adult males, nuptial pads, circumferential grooves on all fingers, a reddish-brown to grayish dorsal coloration, yellow ventral and hidden surfaces, and dark supra-inguinal spots. Molecularly, the new species differs from all congeners by an uncorrected p-distance of > 7% for the 16S rRNA gene fragment. Furthermore, the possession of dark supra-inguinal spots optimizes unambiguously as a synapomorphy for two clades within Strabomantidae. The first clade comprises members of the genus Niceforonia and includes N. brunnea, N. dracula, N. elassodiscus, N. mantipus, N. nigrovittata, N. quilloturo, N. peraccai, four candidate species, and the new species described herein. This clade is referred to as the Niceforonia nigrovittata species group, diagnosed by the presence of dark supra-inguinal spots. By retaining this name, we ensure taxonomic stability and honor the well-established historical use of the nomenclature in literature. The second clade comprises members of the subfamily Holoadeninae, including the genera Bahius, Barycholos, Phyllonastes, and Urkuphryne. A previous study already proposed a phenotypic synapomorphy for the group composed of Phyllonastes + Barycholos. Consequently, the available evidence corroborates the monophyly of both the group comprising these four holoadenine genera and the Phyllonastes + Barycholos group. This congruence provides a robust, nested phylogenetic resolution and precise taxonomic diagnoses, demonstrating how the integration of molecular and phenotypic evidence is a powerful tool for testing evolutionary hypotheses in brachycephaloid frogs.

Key words: Brachycephaloid frogs, Cerro El Inglés, evolution, Key Biodiversity Area (KBA), taxonomy

Living specimens of Niceforonia tempestuosa and N. mantipus.
Dorsal and ventral views of adult males of Niceforonia tempestuosa. A, C. ARUQ-1885, SVL 24.5 mm; B, D. ARUQ-1882, SVL 23.8 mm
and N. mantipus; E, G. Specimen not collected; F, H. ARUQ-1881, SVL 23.5 mm.

Family: Strabomantidae Hedges, Duellman & Heinicke, 2008 

Genus: Niceforonia Goin & Cochran, 1963

Niceforonia tempestuosa sp. nov.

 

Proposed standard Spanish name. Rana de los bosques montanos nublados.

Proposed standard English name. Montane cloud forest frog.

Diagnosis. Niceforonia tempestuosa is diagnosed by the following combination of characters: (1) skin of dorsum smooth with scattered small warts; dorsolateral and \ /-shaped scapular folds present; venter smooth, discoidal fold absent; (2) tympanic membrane differentiated, upright-oval to round; its length 33.3–44.8% of eye length in six adult males and 47% in one adult female; prominent tympanic annulus, its upper edge covered by supratympanic fold, extending only just posterior to tympanic annulus; (3) snout subacuminate in dorsal view, rounded in profile; a fleshy swelling of lip in adult males; canthus rostralis straight; (4) upper eyelid bearing minute tubercles, narrower than IOD (62.0–85.1% IOD); interocular tubercle absent; cranial crest absent; (5) choanae small, ovoid; partially concealed by palatal shelf of maxillary arch; dentigerous ...

Etymology. The specific epithet is the feminine adjective tempestuosa (meaning “stormy”), derived from the Latin noun tempestas (meaning “storm” or “weather”). This name was chosen for local communities as a metaphor for the global climate emergency, referring to the increased frequency and intensity of extreme weather events—symbolized by the “storm”—which represent a growing threat to global biodiversity. The discovery of this new species in the Key Biodiversity Area (KBA) Serranía de los Paraguas underscores the resilience of this biodiversity hotspot amidst the current climate crisis, safeguarded by the persistent efforts of local communities and biodiversity guardians.

Habitat of Niceforonia tempestuosa at the type locality. The habitat is characterized by the presence of large tree buttresses and stilt roots of Wettinia sp. palms, with the forest floor covered by abundant leaf litter.

 Jhon Jairo Ospina-Sarria, Andrés Felipe Rodríguez-Betancourt, Ximena Gómez-Sepúlveda, Iván Darío Morales-Vertel, Valentina Vélez-Franco, Nayely Gallego-Posada, Jose Daniel Herrera-Rengifo, John Edison Grajales Escobar, Irene Ceballos-Castro, Isabella García-Gómez, David Andrés Velásquez-Trujillo, Ayda Susana Ortiz Baez and Javier Mendez Narvaez. 2026. A New Species of Niceforonia (Amphibia, Anura, Strabomantidae) from the Serranía de los Paraguas, Colombia, with the Description of A New phenotypic synapomorphy for the Niceforonia nigrovittata species group. ZooKeys. 1289: 159-184. DOI: doi.org/10.3897/zookeys.1289.198411 [11-08-2026]

[Paleontology • 2026] Spea labreae • A New extinct Pleistocene Species of Spadefoot Toad (Anura: Scaphiopodidae) and comments on the Paleoecology and Paleobiogeography of Anurans from Rancho La Brea, California

Reconstruction of Spea labreae, (dark spadefoots, center) in La Brea Tar Pits compared with Spea hammondii (light spadefoot, left), the species currently present in the area, and the presence of the Mexican burrowing toad (Rhinophrynidae, bottom). 

 Cruz & Lindsey, 2026

 DOI: doi.org/10.1080/02724634.2026.2689465 

Artwork by Arturo Dávalos

ABSTRACT

Fossil amphibians hold great promise for paleoecological, paleoclimatological, and biogeographic studies because of their extreme sensitivity to environmental conditions. However, the study of fossil amphibians to date has been limited, because the fragility of frog, toad, and salamander skeletons means that diagnostic elements are rarely preserved in the fossil record. One unique locality that preserves a robust record of Quaternary amphibians is Rancho La Brea in Los Angeles, California, U.S.A. Here, we describe the amphibian fauna from the Hancock Collection of Rancho La Brea, which contains fossils from ∼45,000 years before present through the Holocene. We document previously unrecognized taxa from this locality, including the Mexican burrowing toad Rhinophrynus sp. and a new species of spadefoot toad, Spea labreae, sp. nov. Using osteological comparison material, we calculate the size of this new species of Spea, which is larger than the extant species in this genus. We analyze the current distribution and fossil record of the genus Spea and the family Rhinophrynidae, documenting changes in geographic ranges of these taxa during the Quaternary. These changes likely resulted from the interplay of deglacial climate changes and topographic complexity in the American Southwest, and may help to predict amphibian responses to climate changes impacting the region today.

Class AMPHIBIA, Linnaeus, 1767

Order ANURA Rafinesque, 1815

Family SCAPHIOPODIDAE Cope, 1865

Genus SPEA Cope, 1866

SPEA LABREAE, sp. nov.

Etymology—The specific epithet refers to La Brea, Spanish for ‘the tar’ which gave rise to the name of Rancho La Brea, the locality where this species is found.

 

Locality, Horizon, and Age—Pit A, Rancho La Brea, Los Angeles, California, Late Pleistocene–Holocene.

 

Reconstruction of Spea labreae, (dark spadefoots, center and right) in La Brea Tar Pits compared with Spea hammondii (light spadefoot, left), the species currently present in the area, and the presence of the Mexican burrowing toad (Rhinophrynidae, bottom).

 Artwork by Arturo Dávalos.

 

J. Alberto Cruz and Emily L. Lindsey. 2026. A New extinct Pleistocene Species of Spadefoot Toad and comments on the Paleoecology and Paleobiogeography of Anurans from Rancho La Brea, California. Journal of Vertebrate Paleontology. e2689465. DOI: doi.org/10.1080/02724634.2026.2689465 [04 Aug 2026]

[Herpetology • 2026] Gracixalus damingshanensis • A New Species of Gracixalus (Anura: Rhacophoridae) from Damingshan National Nature Reserve, Guangxi, China

 

 Gracixalus damingshanensis 

Chen & Li, 2026  

大明山纤树蛙  ||  DOI: doi.org/10.3897/zse.102.186426 

 Photographs by Wei-Cai Chen.

Abstract

A new species of Gracixalus is described from central Guangxi, China. Gracixalus damingshanensis sp. nov. resembles G. jinxiuensis, G. huaping, and G. liusanjieae, but differs from all known congeners by a combination of morphological, molecular, and acoustic characters. Diagnostic characters include SVL 27.3–32.3 mm in males (n = 4) and 32.4–40.1 mm in females (n = 3); rough dorsal skin with scattered tubercles; grayish ventral surfaces with light brown spotting; fingers without webbing; white tubercles along the margins of the upper and lower jaws and on the limbs; absence of vomerine teeth; tibiotarsal articulation reaching the middle of the eye; granular skin on the throat, chest, and belly; heels not meeting when the thighs are held at right angles to the body; a distinct nuptial pad on Finger I; a single internal subgular vocal sac with paired oval openings bordered by gray rims; a golden-brown iris; and advertisement calls containing 2–5 notes. Phylogenetic analyses of mitochondrial 16S rRNA sequences recovered the new species as a distinct lineage sister to the clade comprising G. jinxiuensis and G. huaping. The species is currently known only from evergreen and bamboo forests above 900 m a.s.l. in Damingshan National Nature Reserve.

Key Words: Bioacoustics, morphology, phylogeny, shrub frogs, taxonomy

 

Holotype (NNU202103010) of Gracixalus damingshanensis sp. nov.
 A. Dorsal view; B. Dorsolateral view; C. Ventral view; D. Ventral view of right hand; E. Nuptial pad on Finger I; F. Ventral view of left foot. Photographs by Wei-Cai Chen.

Color variation among paratypes of Gracixalus damingshanensis sp. nov. 
 A1, A2. Dorsal and ventral views of male NNU202103008;
B1, B2. Dorsal and ventral views of male NNU202103009;
C1, C2. Dorsal and ventral views of male NNU202103011;
D1, D2. Dorsal and ventral views of female NNU202103007.

 Photographs by Wei-Cai Chen.

Gracixalus damingshanensis sp. nov. 

Diagnosis. Gracixalus damingshanensis sp. nov. can be distinguished from its congeners by the following combination of characters: (1) SVL 27.3–32.3 mm in males (n = 4) and 32.4–40.1 mm in females (n = 3); (2) dorsal surface rough, with scattered tubercles; (3) throat, chest, and ventral surface grayish with light brown spotting; (4) fingers without webbing; (5) white tubercles along the margins of the upper and lower jaws and on limb surfaces; (6) vomerine teeth absent; (7) tibiotarsal articulation reaching the middle of the eye; (8) skin of throat, chest, and ...

Etymology. The specific epithet ‘damingshanensis’ refers to Damingshan National Nature Reserve in Nanning, Guangxi, China, where the species was discovered. We propose “Damingshan Tree Frog” as the English common name and “Dà Míng Shān Xiān Shù Wā“ (大明山纤树蛙) as the Chinese common name.

Wei-Cai Chen and Peng Li. 2026. A New Species of Gracixalus (Anura, Rhacophoridae) from Damingshan National Nature Reserve, Guangxi, China. Zoosystematics and Evolution. 102(4): 989-1003. DOI: doi.org/10.3897/zse.102.186426