[Herpetology • 2018] Theloderma petilum | ปาดหลังยาวภูสวนทราย (Anura, Rhacophoridae) • A New Country Record for Thailand

Theloderma petilum (Stuart & Heatwole, 2004)

in Phusaensri, Kaewboribut, Phummisutthigoon, et al., 2018. 

Alytes. 36(1-4). alytes-journal.org 

ปาดหลังยาวภูสวนทราย  ||  ku.ac.th

We here report the first finding of a specimen of the treefrog Theloderma petilum (Stuart & Heatwole, 2004) in Thailand. To date, only four other individuals of this species were known from Laos and Vietnam. The female specimen of T. petilum collected at Huay Tap Ko Sod of Baan Huay Nam Phak, Na Haeo District, Loei...

ปาดหลังยาวภูสวนทราย Theloderma petilum (Stuart & Heatwole, 2004) เพศเมีย

Alytes. 36(1-4). alytes-journal.org  

ปาดหลังยาวภูสวนทราย เป็นชนิดสัตว์สะเทินน้ำสะเทินบกที่พบครั้งแรกในประเทศไทย ถือเป็นบันทึกแหล่งการกระจายแหล่งที่ 3 เพิ่มเติมจากที่พบในสาธารณรัฐสังคมนิยมเวียดนาม และสาธารณรัฐประชาธิปไตยประชาชนลาว 

ในประเทศไทยถูกค้นพบโดย คณะวิจัยที่มีประกอบด้วย ผศ. ดร. ประทีป ด้วงแค คณะวนศาสตร์ มหาวิทยาลัยเกษตรศาสตร์ ร่วมกับนักวิชาการจากรมอุทยานแห่งชาติ สัตว์ป่า และพันธุ์พืช และ มหาวิทยาลัยขอนแก่น ได้รับตีพิมพ์ในวารสาร Alytes 36 (1-4) ระหว่างหน้าที่ 289-299 ในปี พ.ศ. 2561

Samret Phusaensri, Thanawhat Kaewboribut, Sukpaporn Phummisutthigoon, Nithina Kaewtongkum, Montri Youjaruen, Pongsathorn Tongpun, Hathaipat Khongcharoensuk, Pechrkawin Nurngsomsri, Chantip Chuaynkern, Prateep Duengkae and Yodchaiy Chuaynkern. 2018. Theloderma petilum (Anura, Rhacophoridae): A New Country Record for Thailand. Alytes. 36(1-4); 289–299.

alytes-journal.org/alytes/alytes-volume-36/theloderma-petilum-anura-rhacophoridae-new-country-record-thailand

การค้นพบพืชชนิดใหม่ของโลก และชนิดสัตว์สะเทินน้ำสะเทินบกครั้งแรกในประเทศไทย

ระหว่างเดือนมิถุนายน ถึงเดือนสิงหาคม พ.ศ. 2561

ของอาจารย์ภาควิชาชีววิทยาป่าไม้ คณะวนศาสตร์ มหาวิทยาลัยเกษตรศาสตร์

ku.ac.th/web2012/index.php?c=adms&m=seltab_th&time=20180810094001&page=&page1=&load=tab&lang=thai&ip=10&id=3363&id1=87

 forest.ku.ac.th/webdev3/newsuploadfile/921-2018-08-10_114907.pdf

Abstract: A small rhacophorid, Philautus petilus known from only the female holotype, is recorded for the first time outside of the type locality in Laos. Three specimens, containing the first known males of the species, were collected from Muong Nhe Nature Reserve in Dien Bien Province, northwestern Vietnam. The Vietnamese specimens are identified as P. petilus based on morphological similarities with the holotype from Laos. In addition, our molecular data verify the transfer of this species from Philautus to the genus Theloderma.

Keywords: Philautus, Theloderma, New record, Phylogeny, Taxonomy, Muong Nhe Nature Reserve, Dien Bien Province

 Tao Thien Nguyen, Dzung Trung Le, Son Hung Lan Nguyen, Masafumi Matsui and Truong Quang Nguyen. 2014. First Record of Philautus petilus Stuart and Heatwole, 2004 (Amphibia: Anura: Rhacophoridae) from Vietnam and Its Phylogenetic Position. Current Herpetology. 33(2); 112-120. 

 researchgate.net/publication/280288741_First_Record_of_Philautus_petilus

DOI:  10.5358/hsj.33.112

repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/216855/1/hsj.33.112.pdf

Bryan L. Stuart and Harold Heatwole. 2004. A New Philautus (Amphibia: Rhacophoridae) from northern Laos. Asiatic Herpetological Research. 10: 17-21. 

 asiatic-herpetological.org/Archive.htm
 researchgate.net/publication/228506727_A_new_Philautus_from_northern_Laos

[Herpetology • 2018] Theloderma auratum • A New Species of the Genus Theloderma Tschudi, 1838 (Anura: Rhacophoridae) from Tay Nguyen Plateau, central Vietnam

Theloderma auratum 

Poyarkov, Kropachev, Gogoleva & Orlov, 2018

 DOI: 10.24272/j.issn.2095-8137.2018.018 

ABSTRACT 

A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloderma auratum sp. nov., is distinguishable from its congeners and other small rhacophorid species based on a combination of the following morphological attributes: (1) bony ridges on head absent; (2) smooth skin completely lacking calcified warts or asperities; (3) pointed elongated tapering snout; (4) vocal opening in males absent; (5) vomerine teeth absent; (6) males of small body size (SVL 21.8–26.4 mm); (7) head longer than wide; ED/SVL ratio 13%–15%; ESL/SVL ratio 16%–20%; (8) small tympanum (TD/EL ratio 50%–60%) with few tiny tubercles; (9) supratympanic fold absent; (10) ventral surfaces completely smooth; (11) webbing between fingers absent; (12) outer and inner metacarpal tubercles present, supernumerary metacarpal tubercle single, medial, oval in shape; (13) toes half-webbed: I 2–2¼ II 1½–2¾ III 2–3¼ IV 3–1½ V; (14) inner metatarsal tubercle present, oval; outer metatarsal tubercle absent; (15) iris bicolored; (16) dorsal surfaces golden-yellow with sparse golden-orange speckling or reticulations and few small dark-brown spots; (17) lateral sides of head and body with wide dark reddish-brown to black lateral stripes, clearly separated from lighter dorsal coloration by straight contrasting edge; (18) ventral surfaces of body, throat, and chest greyish-blue with indistinct brown confluent blotches; (19) upper eyelids with few (3–5) very small flat reddish superciliary tubercles; (20) limbs dorsally reddish-brown, ventrally brown with small bluish-white speckles. The new species is also distinct from all congeners in 12S rRNA to 16S rRNA mitochondrial DNA fragment sequences (uncorrected genetic distance P>8.9%). Advertisement call and tadpole morphology of the new species are described. Our molecular data showed Theloderma auratum sp. nov. to be a sister species of Th. palliatum from Langbian Plateau in southern Vietnam. 

Keywords: Theloderma auratum sp. nov.; mtDNA phylogeny; 12S rRNA; 16S rRNA; Kon Tum; Gia Lai; Endemism; Taxonomy

 Figure 4 Dorsolateral view of male holotype of Theloderma auratum sp. nov. (ZMMU A-5828) in life (in situ) 

Photo by Nikolay A. Poyarkov

Theloderma auratum sp. nov.

....

Etymology: The specific name “auratum” is a Latin adjective in the nominative singular (neutral gender), derived from Latin “aurum” for “gold”, referring to the golden-yellowish dorsal coloration of the new species.

Recommended vernacular name: We recommend the following common name in English: Golden Bug-Eyed Frog. Recommended vernacular name in Vietnamese: ´Êch Cây Sân Vàng.

Distribution and biogeography: The known distributions of Theloderma auratum sp. nov. and its sister species Th. palliatum are shown in Figure 1. To date, the new species is known from montane evergreen tropical forests of Tay Nguyen Plateau in the central Annamite (Truong Son) Mountains, and has been recorded in Gia Lai, Kon Tum, and Thua Thien-Hue provinces. It is anticipated that Theloderma auratum sp. nov. also occurs in the adjacent montane forests of Tay Nguyen Plateau; in particular, records from Quang Nam Province of Vietnam and Xekong Province of Laos are anticipated. 

Natural history notes: Our knowledge on the biology of Theloderma auratum sp. nov. is scarce. The new species was recorded in primary polydominant tropical montane evergreen forests of Tay Nguyen Plateau at elevations ranging from 800 to 1 400 m a.s.l.. Animals were recorded only in patches of primary undisturbed forest with complete multi-layered canopy and heavy undergrowth, suggesting the new species is a strict forest-dwelling specialist. At the type locality in Kon Chu Rang Nature Reserve (Gia Lai Province), the forest where the new species was recorded is dominated by large trees of the families Podocarpaceae (Dacrydium elatum, Dacrycarpus imbricatus), Magnoliaceae, Burseraceae (Canarium sp.), Myrtaceae (Syzygium sp.), Hamamelidaceae (Simingtonia sp.), Lauraceae (Litsia sp.), Rhodoliaceae (Rhodolia sp.), Fagaceae, Sterculiaceae (Scaphium sp.) (Figure 10). ....

Nikolay A. Poyarkov, Jr., Ivan I. Kropachev, Svetlana S. Gogoleva and Nikolai L. Orlov. 2018. A New Species of the Genus Theloderma Tschudi, 1838 (Amphibia: Anura: Rhacophoridae) from Tay Nguyen Plateau, central Vietnam. Zoological Research. 39(3); 156-180.   DOI: 10.24272/j.issn.2095-8137.2018.018

  

[Herpetology • 2017] Theloderma pyaukkya • A New Cryptic Species of the Theloderma asperum Complex (Anura: Rhacophoridae) from Myanmar

Theloderma pyaukkya Dever, 2017

DOI: 10.1670/17-026  || @JADever

Abstract

I describe a new species of Theloderma from two regions in Myanmar (Chin State in western Myanmar and Kachin State in northern Myanmar). Highly similar in appearance and size to Theloderma albopunctatum and Theloderma asperum, the new species differs by the presence of small, bilateral vocal sac openings absent in T. albopunctatum and T. asperum. Molecular phylogenetic analysis from two mitochondrial and four nuclear gene fragments infers that individuals are members of a unique genetic lineage within the T. asperum Complex.

FIG. 6. Theloderma pyaukkya sp. nov., paratype CAS 234869, adult male, dorsolateral view. 

Theloderma pyaukkya sp. nov. 

Burmese Camouflaged Tree Frog

Etymology.— Specific epithet pyaukkya (pronounced pee-ew-cha) is Burmese for camouflaged, which reflects the frog’s cryptic coloration.

Jennifer A. Dever. 2017. A New Cryptic Species of the Theloderma asperum Complex (Anura: Rhacophoridae) from Myanmar. Journal of Herpetology. 51(3); 425–436.  DOI: 10.1670/17-026

ResearchGate.net/publication/318757149_A_New_Cryptic_Species_of_the_Theloderma_asperum_Complex_Anura_Rhacophoridae_from_Myanmar

twitter.com/@JADever/status/844305426069913600

    

[Herpetology • 2017] The Taxonomic Status and Distribution Range of Six Theloderma Species (Anura: Rhacophoridae) with A New Record in China

Theloderma moloch (Annandale, 1912)

Yunnan, China, 1000 m elevation.

Photo by Mian Hou 

Abstract

The problems of identification, number and distribution of Theloderma species living in China are discussed on the base of new original morphological and molecular data collected during the last years. According to the author’s results there are six known Theloderma species living in China: Theloderma albopunctatum (Liu et Hu, 1962), Theloderma baibungense Jiang, Fei et Huang, 2007, Theloderma bicolor (Bourret, 1937), Theloderma corticale (Boulenger, 1903), Theloderma moloch (Annandale, 1912), and Theloderma rhododiscus Liu et Hu, 1962.

Keywords: taxonomy; distribution; Theloderma; new records; China


Theloderma corticale (Boulenger, 1903)

English name. Tonkin Bug-eyed Frog, Kwangsi Warty Treefrog.

Etymology. The specific epithet is derived from Latin “cortex,” genit. cortices or corticulus, means bark.
Distribution. Known from south China, Laos, and Vietnam.

Theloderma bicolor (Bourret, 1937)

English name. Chapa Bug-eyed Frog. 

Etymology. the specific epithet is derived from Latin “bis,” means two, twice; and color is from Latin “color,” as hue, tint or complexion.
Distribution. Northwest to central Vietnam; southwest China.


 Theloderma moloch  (Annandale, 1912)

Theloderma asperum species group, such as Theloderma albopunctatum (southern China to central Indochina), Theloderma baibungense (SE Himalaya) and T. asperum (south Indochina to Malaysian Peninsula).

Theloderma rhododiscus  Liu et Hu, 1962

....

According to above discussion, currently there are six known Theloderma species in China, such as Theloderma albopunctatum (Liu et Hu, 1962), Theloderma baibungense Jiang, Fei et Huang, 2007, Theloderma bicolor (Bourret, 1937), Theloderma corticale (Boulenger, 1903), Theloderma moloch (Annandale, 1912), and Theloderma rhododiscus Liu et Hu, 1962 

Hou Mian, Yu Guo-Hua, Chen Hong-man, Liao Chang-Le, Zhang Li, Chen Jin, Li Pi-Peng and Nikolai L. Orlov. 2017. The Taxonomic Status and Distribution Range of Six Theloderma Species (Anura: Rhacophoridae) with A New Record in China.
 Russian Journal of Herpetology. 24(2); 91-127. 

ResearchGate.net/project/Theloderma-Taxonomy-of-China
 Theloderma in China (Anura: Rhacophoridae); Taxonomic Status and Distribution Range 

[Herpetology • 2016] Theloderma lacustrinum • A New Small-sized Theloderma (Anura: Rhacophoridae) from Laos

Theloderma lacustrinum 

Sivongxay, Davankham, Phimmachak, Phoumixay & Stuart, 2016

  DOI: 10.11646/zootaxa.4147.4.5 

Abstract

A new species of the rhacophorid frog genus Theloderma is described from the forested shoreline of the Nam Lik Reservoir, Vientiane Province, Laos. The new species differs from its congeners by having the combination of males with SVL 17.0–20.6; pearly asperities on dorsum; no vomerine teeth; disc diameter of finger III ca. 40% of tympanum diameter; uniformly gray venter; light brown dorsum with darker brown and black markings; and a uniformly bronze iris with small black reticulations. Molecular phylogenetic analysis of mitochondrial DNA sequence data infers that the new species is most closely related to T. lateriticum from northern Vietnam. Evidence for the monophyly of Theloderma is reviewed.

Keywords: Amphibia, Nam Lik Reservoir, Frankixalus, Nasutixalus, Theloderma lateriticum, Theloderma moloch

Etymology. The specific epithet taken from lacustrinus L., of lakes, in reference to Nam Lik Reservoir, a large, man-made reservoir formed by a hydropower dam that has inundated the vicinity of the type locality.

Diagnosis. Assigned to the genus Theloderma on the basis of molecular data (Fig. 2). A very small-sized (second smallest known) species of Theloderma having the combination of males with SVL 17.0–20.6; pearly asperities on dorsum; no vomerine teeth; disc diameter of finger III ca. 40% of tympanum diameter; uniformly gray venter; light brown dorsum with darker brown and black markings; and a uniformly bronze iris with small black reticulations.

Distribution, natural history, and conservation. Theloderma lacustrinum sp. nov. is currently known only from the type and paratype localities (Fig. 3), where it was collected at night (1820–2215 h) on leaves 30–80 cm above the ground near two rocky streams (292–361 m elev.) in semi-evergreen forest that flow into the Nam Lik Reservoir (Fig. 1). The two known localities are approximately 5.7 air-km apart. We did not observe the new species at these localities during visits in April, July, and September 2015, suggesting that it may be seasonally active or most detectable during cooler, drier months (e.g., at lower heights in the forest canopy). The vicinity of the two known localities had not been surveyed for amphibians prior to construction of the hydroelectric dam, and thus it is not known how inundation from the reservoir may have impacted the range of the species.

Sivongxay, Niane, Monekham Davankham, Somphouthone Phimmachak, Keochay Phoumixay & Bryan L. Stuart. 2016. A New Small-sized Theloderma (Anura: Rhacophoridae) from Laos. Zootaxa.  4147(4); 433–442.  DOI: 10.11646/zootaxa.4147.4.5

http://www.bryanlstuart.com/site/Publications_files/Sivongxay%20et%20al.%202016.pdf

  

[Herpetology • 2015] Rediscovery and Redescription of Theloderma phrynoderma (Ahl, 1927) (Anura: Rhacophoridae) from Myanmar

Theloderma phrynoderma (Ahl, 1927), adult male 

 DOI: 10.1643/CH-14-130  

 ResearchGate.net/publication/277884659

Theloderma is a widely distributed yet little-known genus of camouflaged tree frogs found throughout Southeast Asia. One member, T. phrynoderma, known only from the moist evergreen forest of the Karen Hills of Myanmar, is redescribed from two recently collected specimens and examination of type specimens. To date the only information available about T. phrynoderma is Boulenger’s brief 1893 description of two type specimens collected in 1888, and phylogenetic analyses to test its placement among other species of Theloderma is lacking due to an absence of specimens. In the present study, we compared two individuals collected in 2009 and 2010 from the Tanintharyi Nature Reserve to the type specimens of T. phrynoderma and proposed that they are also members of this species. We then used two mitochondrial genes (12S and 16S rRNA) and two nuclear genes (rhodopsin and tyrosinase) to infer the phylogenetic relationship of the putative T. phrynoderma to other members of Rhacophoridae, with a special emphasis on Theloderma. The recently collected individuals are of the same species within Theloderma but distinct from all other DNA sequenced congeners. The species redescription is based on a comparison of the newly found reference specimens with the lectotype and paralectotype. In addition, using a combination of morphological characters we provide a more complete diagnosis. The species is distinct from other congeners by a combination of the following characters: a mid-body size (female 44 mm SVL; male mean 41.3 mm SVL); tympanum diameter to eye diameter (70%); partial webbing between fingers; rugose skin with clumped, white-tipped calcified tubercles throughout the dorsal surface; webbing between fingers; distinct darker brown inverted V-marking between its shoulders; absence of vomerine teeth; and absence of vocal sacs.

Redescription of Theloderma phrynoderma (Ahl, 1927)
Figures 6–10; Table 3

Phrynoderma asperum Boulenger, 1893:342.

Rhacophorus phrynoderma Ahl, 1927:47.

Rhacophorus (Phrynoderma) phrynoderma Ahl, 1931:60.

Rhacophorus (Rhacophorus) leprosus phrynoderma Wolf, 1936:158.

Theloderma phrynoderma Inger, 1985:550.

Geographic distribution.—This species is known from two localities in Myanmar (Fig. 1). The lectotype and paralectotype were collected in Thao, at an elevation of 1,300–1,400 a.s.l. The collected reference specimens were collected at a lower elevation of 59–82 a.s.l. from two sites in the TNR of northern Tanintharyi Division. Although separated by approximately 550 km, the Thao collection locality and Tanintharyi collection localities lie within a contiguous tropical-subtropical moist evergreen forest ecoregion (Olson et al., 2001).

Dever, Jennifer A., Hai Nguyen and Jeffery A. Wilkinson. 2015. Rediscovery and Redescription of Theloderma phrynoderma (Ahl, 1927) (Anura: Rhacophoridae) from Myanmar. Copeia. 103 (2): 402-415. DOI: 10.1643/CH-14-130

 ResearchGate.net/publication/277884659_Rediscovery_and_Redescription_of_Theloderma_phrynoderma_(Ahl_1927)_(Anura_Rhacophoridae)_from_Myanmar

[Herpetology • 2016] Nasutixalus medogensis • A New Genus and Species of Treefrog (Anura, Rhacophoridae) from Medog, southeastern Tibet, China

墨脱棱鼻树蛙 |  Nasutixalus medogensis

  Jiang, Yan, Wang & Che, 2016

photo: Ke JIANG [蒋珂] || DOI: 10.13918/j.issn.2095-8137.2016.1.15

Abstract

A new genus and species of threefrog is described from Medog, southeastern Tibet, China based on morphological and phylogenetic data. The new genus can be distinguished from other treefrog genera by the following combination of characters: (1) body size moderate, 45.0 mm in male; (2) snout rounded; (3) canthus rostralis obtuse and raised prominently, forming a ridge from nostril to anterior corner of eyes; (4) web rudimentary on fingers; (5) web moderately developed on toes; (6) phalange "Y" shaped, visible from dorsal side of fingers and toes; (7) skin of dorsal surfaces relatively smooth, scatted with small tubercles; (8) iris with a pale yellow, "X" shaped pattern of pigmentation.

Key words: Taxonomy, New genus, New species, Theloderma moloch, Nasutixalus medogensis sp. nov.

Phylogenetic analysis: Currently  recognized  genera  of  the  family  Rhacophoridae  were recovered  as  monophyletic  groups  in  our  phylogenetic  analysis  (Figure 1). However, similar to previous studies (Li et al., 2009), our data  cannot  resolve  phylogenetic  relationships  among  different  genera.   The Medog treefrog   was   clustered   and   share   the   haplotype  with  a  formerly  identified  T. moloch  (specimen  voucher  number  6255  RAO)  in  Li  et  al.  (2009),  and  both  of  them  form  a  distinct clade from all other species of genus Theloderma, including the  true T. moloch.  Such  result  is  similar  to  the  phylogenetic  topography recovered in Li et al. (2013) using more datasets. Therefore,   according   to   a   combination   of   morphological   characters  and  phylogenetic  data  of  mitochondrial  genes,  we  conclude that the male treefrog specimen (KIZ016395) consisted an  independent  evolutionary  lineage  and  concordant  evidence  confirm species status (Hou et al., 2014; Wu & Murphy, 2015), which is described as a new species and a new genus in family Rhacophoridae.  

Nasutixalus gen. nov. Jiang, Yan, Wang and Che 

Type species: Nasutixalus medogensis sp. nov. 

Diagnosis: (1)  Body  size  moderate  (45.0  mm  in  male);  (2)  snout  rounded;  (3)  canthus  rostralis  obtuse  and  raised  prominently,  forming  a  ridge  from  nostril  to  anterior  corner  of  eyes;  (4)  web  rudimentary on fingers; (5) web moderately developed on toes; (6) phalange “Y” shaped, visible from dorsal side of fingers and toes;  (7)  skin  of  dorsal  surfaces  relatively  smooth,  scatted  with  small tubercles; (8) iris with a pale yellow, “X” shaped pattern of pigmentation, especially distinct in preservative. 

Distribution: Currently   known   only   from   the   type   locality,   Medog, Tibet, China.

Etymology: The generic nomen Nasutixalus is derived from the Latin adjective nasutus (“large-nosed” in English), means the prominent ridge from nostril to the anterior corner of eye, and ixalus, a common generic root for treefrogs. We suggest the common name of the new genus be “ridged-nose treefrog” in English, and “Leng Bi Shu Wa” (棱鼻树蛙) in Chinese.

Content: The new genus currently contains a single species, Nasutixalus medogensis sp. nov. which is described below.

Nasutixalus  medogensis  sp.  nov.  Jiang,  Wang,  Yan  and  Che (Figures 2-4) 

Synonyms: Thermoderma moloch: Li et al., 2009 

Holotype: KIZ 016395, adult male, collected from Gelin (N29.21665°, E95.17571°, elevation 1 619 m), Beibeng, Medog, southeastern Tibet, China, on 28 April  2015, by Ke JIANG.

Etymology The species name “medogensis” is named after the type locality, Medog, Tibet, China. According to the Latin name, we suggest the  English  common  name  as  “Medog  Ridged-nose  Treefrog”,  and  the  Chinese  formal  name  as  “Muo  Tuo  Leng  Bi  Shu  Wa”  (墨脱棱鼻树蛙).

Ke JIANG, Fang YAN, Kai WANG, Da-Hu ZOU, Cheng LI and Jing CHE. 2016. A New Genus and Species of Treefrog from Medog, southeastern Tibet, China (Anura, Rhacophoridae).
ZOOLOGICAL RESEARCH. 37(1); 15-20.  DOI: 10.13918/j.issn.2095-8137.2016.1.15

ResearchGate.net/publication/291521962_A_new_genus_and_species_of_treefrog_from_Medog_southeastern_Tibet_China_Anura_Rhacophoridae

藏东南发现树蛙科新属新种 

就在今年，包括笔者在内的几位研究人员一道，在西藏东南部墨脱热带雨林中发现一类全新的树蛙物种！通过DNA和形态比较，我们中国的两栖爬行动物学者确定这不仅仅是一个新物种，它更代表一个从未被描述过的新属！

http://www.ikexue.org/archives/37919

[Herpetology • 2015] Sorting Out Moss Frogs: mtDNA Data on Taxonomic Diversity and Phylogenetic Relationships of the Indochinese Species of the Genus Theloderma (Anura, Rhacophoridae)

Fig. 1. Theloderma species of Indochina and Thailand.
a, Th. horridum (Thailand, Satun Province, Tha Le Ban N. P., photo N. A. Poyarkov); b, Th. stellatum (Thailand, Uthai Thani Province, Hauy Kha Khaeng W. S.; photo P. Pawangkhanant); c, Th. cf. stellatum [described below as Theloderma vietnamense sp. nov.] (Vietnam, Kon Tum Province, Kon Plong); d, Th. pictum (Thailand, Satun Province, Tha LeBan N. P.; photo P. Pawangkhanant); e, Th. licin (Thailand, Satun Province, Tha Le Ban N. P., photo P. Pawangkhanant); f, Th. cf. asperum, northern population (Vietnam, Vinh Phuc Province, Tam Dao; photo N. L. Orlov); g, Th. cf. asperum, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); h, Th. asperum (Thailand, Kamphaeng Phet Province, photo P. Pawangkhanant); i, Th. ryabovi, male (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); j, Th. ryabovi, female (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); k, Th. bicolor (Vietnam, Lao Cai, Fan Si Pan Mt.; photo N. L. Orlov); l, Th. corticale (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); m, Th. gordoni, northern population (Vietnam, Lao Cai, Sa Pa; photo N. L. Orlov); n, Th. gordoni, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); o, Th. lateriticum (Vietnam, Bac Giang; photo Nguyen Thien Tao); p, Th. rhododiscum (China, Yunnan; photo N. L. Orlov); q, Th. palliatum (Vietnam, Lam Dong Province, Bi Doup; photo E. A. Galoyan); r, Th. chuyangsinense (Vietnam, Dak Lak Province, Chu Yang Sin; photo N. A. Poyarkov); s, Th. truongsonense (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); t, Th. bambusicola (Vietnam, Lam Dong Province, Cat Loc; photo N. A. Poyarkov); u, Th. cf. nebulosum (Vietnam, Kon Tum Province; photo N. L. Orlov); v, Th. cf. truongsonense (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); w, (Vietnam, Khanh Hoa Province, Hon Ba; photo A. B. Vassilieva); x, Th. petilum (Vietnam, Dien Bien Province, Muong Nhe; photo Le Trung Dung).

N. A. Poyarkov, N. L. Orlov, A. V. Moiseeva, et al. 2015. Russian Journal of Herpetology. 22(4) 

Abstract

We discuss phylogenetic relationships and taxonomic diversity of the rhacophorid frogs of the genus Theloderma in sight of the novel phylogenetic data obtained from the Bayesian analysis of the up to 1987 bp length fragment of mtDNA (12S rRNA, tRNAval, and 16S rRNA) from the 90 specimens of 21 nominal species of Theloderma and 3 species of Nyctixalus. Our data suggest monophyly of the tribe Nyctixalini, including Th. moloch, and indicate deep divergence between the three major clades: Th. horridum + Th. stellatum group, Nyctixalus and the rest of the Theloderma species (Theloderma sensu stricto). We establish new subgenus Stelladerma subgen. nov. for Th. horridum + Th. stellatum group and discuss provisional taxonomy of Nyctixalini. We also indicate that the taxonomic status of the certain Indochinese Theloderma requires reassessment. In particular, our data suggest deep divergence between Malayan and Indochinese taxa of Th. asperum group and indicate non-monophyly of Th. asperum sensu lato; we resurrect the name Th. albopunctatum (Liu et Hu, 1962) for the Indochinese species. We provide molecular evidence for synonimization of Th. chuyangsinense Orlov et al., 2012 with Th. palliatum Rowley et al., 2011; as well as morphological and genetic evidence for syninomization of Th. bambusicola Orlov et al., 2012 with Th. laeve (Smith, 1924). We indicate a deep morphological and genetic differentiation within the Th. truongsonense (Orlov et Ho, 2005) complex. Finally, we report on the deep divergence within Th. stellatum Taylor, 1962 from eastern Thailand, southern Cambodia, and Vietnam, and describe a new species, Theloderma vietnamense sp. nov., based on morphological, acoustic and genetic lines of evidence.

Keywords: mitochondrial DNA; molecular phylogeny; sequence divergence; morphology; morphometrics; advertisement call; cryptic species; Vietnam

Fig. 1. Theloderma species of Indochina and Thailand.
a, Th. horridum (Thailand, Satun Province, Tha Le Ban N. P., photo N. A. Poyarkov); b, Th. stellatum (Thailand, Uthai Thani Province, Hauy Kha Khaeng W. S.; photo P. Pawangkhanant); c, Th. cf. stellatum [described below as Theloderma vietnamense sp. nov.] (Vietnam, Kon Tum Province, Kon Plong); d, Th. pictum (Thailand, Satun Province, Tha LeBan N. P.; photo P. Pawangkhanant); e, Th. licin (Thailand, Satun Province, Tha Le Ban N. P., photo P. Pawangkhanant); f, Th. cf. asperum, northern population (Vietnam, Vinh Phuc Province, Tam Dao; photo N. L. Orlov); g, Th. cf. asperum, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); h, Th. asperum (Thailand, Kamphaeng Phet Province, photo P. Pawangkhanant); i, Th. ryabovi, male (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); j, Th. ryabovi, female (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); k, Th. bicolor (Vietnam, Lao Cai, Fan Si Pan Mt.; photo N. L. Orlov); l, Th. corticale (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); m, Th. gordoni, northern population (Vietnam, Lao Cai, Sa Pa; photo N. L. Orlov); n, Th. gordoni, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); o, Th. lateriticum (Vietnam, Bac Giang; photo Nguyen Thien Tao); p, Th. rhododiscum (China, Yunnan; photo N. L. Orlov); q, Th. palliatum (Vietnam, Lam Dong Province, Bi Doup; photo E. A. Galoyan); r, Th. chuyangsinense (Vietnam, Dak Lak Province, Chu Yang Sin; photo N. A. Poyarkov); s, Th. truongsonense (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); t, Th. bambusicola (Vietnam, Lam Dong Province, Cat Loc; photo N. A. Poyarkov); u, Th. cf. nebulosum (Vietnam, Kon Tum Province; photo N. L. Orlov); v, Th. cf. truongsonense (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); w, (Vietnam, Khanh Hoa Province, Hon Ba; photo A. B. Vassilieva); x, Th. petilum (Vietnam, Dien Bien Province, Muong Nhe; photo Le Trung Dung).

N. A. Poyarkov, N. L. Orlov, A. V. Moiseeva, et al. 2015.
  Russian Journal of Herpetology. 22(4)

Nikolay A. Poyarkov, Jr., Nikolai L. Orlov, Anna V. Moiseeva, Parinya Pawangkhanant, Thiti Ruangsuwan, Anna B. Vassilieva, Eduard A. Galoyan, Tao Thien Nguyen and Svetlana S. Gogoleva. 2015. Sorting Out Moss Frogs: mtDNA Data on Taxonomic Diversity and Phylogenetic Relationships of the Indochinese Species of the Genus Theloderma (Anura, Rhacophoridae). Russian Journal of Herpetology. 22(4): 241–280 

ResearchGate.net/publication/287330801_Sorting_out_moss_frogs_mtDNA_data_on_taxonomic_diversity_and_phylogenetic_relationships_of_the_Indochinese_species_of_the_genus_Theloderma_Anura_Rhacophoridae

Nguyen Quang Truong and Nguyen Vu Khoi. 2008. First record of Theloderma stellatum Taylor, 1962 from Phu Quoc Island, Kien Giang Province, southern Vietnam. Herpetology Notes. 1: 61-62 

HerpetologyNotes.seh-herpetology.org/Volume1_PDFs/Nguyen_Herpetology_Notes_Volume1_pages61-62.pdf

Fig. 1. Theloderma species of Indochina and Thailand.
  a, Th. horridum (Thailand, Satun Province, Tha Le Ban N. P., photo N. A. Poyarkov); b, Th. stellatum (Thailand, Uthai Thani Province, Hauy Kha Khaeng W. S.; photo P. Pawangkhanant); c, Th. cf. stellatum [described below as Theloderma vietnamense sp. nov.] (Vietnam, Kon Tum Province, Kon Plong); d, Th. pictum (Thailand, Satun Province, Tha LeBan N. P.; photo P. Pawangkhanant); e, Th. licin (Thailand, Satun Province, Tha Le Ban N. P., photo P. Pawangkhanant); f, Th. cf. asperum, northern population (Vietnam, Vinh Phuc Province, Tam Dao; photo N. L. Orlov); g, Th. cf. asperum, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); h, Th. asperum (Thailand, Kamphaeng Phet Province, photo P. Pawangkhanant); i, Th. ryabovi, male (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); j, Th. ryabovi, female (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); k, Th. bicolor (Vietnam, Lao Cai, Fan Si Pan Mt.; photo N. L. Orlov); l, Th. corticale (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); m, Th. gordoni, northern population (Vietnam, Lao Cai, Sa Pa; photo N. L. Orlov); n, Th. gordoni, southern population (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); o, Th. lateriticum (Vietnam, Bac Giang; photo Nguyen Thien Tao); p, Th. rhododiscum (China, Yunnan; photo N. L. Orlov); q, Th. palliatum (Vietnam, Lam Dong Province, Bi Doup; photo E. A. Galoyan); r, Th. chuyangsinense (Vietnam, Dak Lak Province, Chu Yang Sin; photo N. A. Poyarkov); s, Th. truongsonense (Vietnam, Quang Tri Province, Ban Cup; photo N. L. Orlov); t, Th. bambusicola (Vietnam, Lam Dong Province, Cat Loc; photo N. A. Poyarkov); u, Th. cf. nebulosum (Vietnam, Kon Tum Province; photo N. L. Orlov); v, Th. cf. truongsonense (Vietnam, Kon Tum Province, Kon Plong; photo N. L. Orlov); w, (Vietnam, Khanh Hoa Province, Hon Ba; photo A. B. Vassilieva); x, Th. petilum (Vietnam, Dien Bien Province, Muong Nhe; photo Le Trung Dung).

[Herpetology • 2013] Philautus nianeae • A New Philautus (Anura: Rhacophoridae) from northern Laos allied to P. abditus from central Vietnam

Philautus nianeae

Stuart, Phimmachak, Seateun &  Sheridan 2013

Abstract

The small rhacophorid frog Philautus abditus is geographically restricted to central Vietnam and adjacent Cambodia. Our fieldwork in northern Laos resulted in the discovery of a Philautus species that very closely resembles P. abditus, but is at least 330 km from the nearest known locality of that species. The Laos population differs from P. abditus in mitochondrial DNA and coloration, and is described here as a new species. Philautus nianeae sp. nov. is distinguished from its congeners by having the combination of a hidden tympanum; no nuptial pads; smooth skin; large black spots on the hidden surfaces of the hind limbs; light venter with dark spotting; and a bronze iris. A second species of Philautus from northern Laos, P. petilus, is transferred on the basis of morphology to the genus Theloderma.

Keywords: Laos; new species; Philautus abditus; Philautus petilus; Rhacophoridae

Etymology. The specific epithet is a matronym for Dr. Niane Sivongxay, Professor of Biology at the National University of Laos, co-collector of the species, and cherished friend and colleague of the authors.

Distribution and natural history. Philautus nianeae is known from three localities in Vientiane, Bolikhamxay, and Khammouan Provinces in northern Laos (Figure 1). In Vientiane Province, males were found during mid-May calling at night (2015–2130 h) on vegetation within 2 m of the ground and within 5 m of streams and riverbanks in disturbed semi-evergreen forest, sometimes mixed with bamboo, at 490–548 m elevation. In Bolikhamxay Province, a female and three juveniles were found during early March on rainy nights (1912–1952 h) 1 m above the ground on sapling leaves and palm fronds within 3 m of small rocky streams in semi-evergreen forest at 471–488 m elevation. In Khammouan Province, males were found during mid-May calling at night (2000–2110 h) on vegetation 1.5–4 m above the ground in semi-evergreen mixed with pine forest near the edge of open grassland at 972–979 m elevation. The Nam Ngum River at the type locality in Vientiane Province is under concession for a hydroelectric power project, making the persistence of the species at the type locality uncertain.

Stuart, Bryan L., Somphouthone Phimmachak, Sengvilay Seateun & Jennifer A. Sheridan. 2013. A New Philautus (Anura: Rhacophoridae) from northern Laos allied to P. abditus Inger, Orlov & Darevsky, 1999. Zootaxa. 3745(1): 73-83.

http://biotaxa.org/Zootaxa/article/view/zootaxa.3745.1.6

http://www.bryanlstuart.com/site/Publications_files/Stuart%20et%20al.%202013%20Zootaxa.pdf

[Herpetology • 2011] Theloderma nebulosum & T. palliatum • Two new species of Theloderma (Anura: Rhacophoridae) from montane forest in Vietnam

Abstract

Two new species of rhacophorid frogs in the genus Theloderma are described from high-elevation montane forest on the Kon Tum and Langbian Plateaus in Vietnam, on the basis of morphological and molecular evidence. Both new species are differentiated morphologically from their congeners by their small body size (<30 mm SVL); absence of vomerine teeth; rugose skin texture with minute, calcified dorsal asperities; no webbing on hands; brownish dorsum; and a bicoloured iris (pale gold in upper third and reddish brown in lower two-thirds). Molecular evidence supports that both new species are members of genus Theloderma, and are distinct from all congeners sampled (uncorrected sequence divergences at the 16S rRNA gene of >8.5% for all Theloderma for which homologous 16S rRNA sequences are available). The new species are differentiated from each other by dorsal pattern, extent of dorsal asperities, and by mitochondrial DNA.

Key words: Anura, Rhacophoridae, Southeast Asia, Vietnam

Cloaked Moss Frog Theloderma palliatum 

| The species, known only from high-elevation forest on the Langbian Plateau in Vietnam, is named after its ability to change from a dull brownish colour to reveal this bold, high-contrast pattern.

Photo: Jodi J. L. Rowley | http://australianmuseum.net.au/

Misty Moss Frog Theloderma nebulosum 

| The species is named after its habitat- mist-shrouded forest on the moutains of the Kon Tum Plateau, Vietnam.

 Photo: Jodi J. L. Rowley | http://australianmuseum.net.au

 Jodi J L Rowley, Le T. T. D, Huy Duc Hoang, Dau Q. V. and Thuy Cao. 2011. Two new species of Theloderma (Anura: Rhacophoridae) from Vietnam. Zootaxa.   3098: 1–20

researchgate.net/publication/223916590_Two_new_species_of_Theloderma_Anura_Rhacophoridae_from_Vietnam

http://www.mapress.com/zootaxa/2011/f/z03098p020f.pdf

http://australianmuseum.net.au/BlogPost/Science-Bytes/Two-new-species-of-moss-frog-discovered

[Herpetology • 2004] Philautus petilus • A New Philautus (Rhacophoridae) from northern Laos

Philautus petilus Stuart & Heatwole, 2004

Figure 1B. The adult female holotype (FMNH 257902) of Philautus petilus sp. nov.

Photograph by Bryan L. Stuart.

Abstract

A new Philautus is described from Phou Dendin National Biodiversity Conservation Area in northern Laos. Philautus petilus sp. nov. is most remarkable by having a very slender, elongate habitus. Other distinguishing characteristics include having a tympanum diameter 80% of the eye diameter, white asperities on the dorsum, and distinctive coloration consisting of a soft yellow-beige dorsolateral surface with broken black stripes posteriorly, a lavender wash on dorsal surface of limbs, upper lip, and sides, a black stripe below edge of canthus extending from snout tip to flanks near level of mid-body, and a black spot equal in diameter to the tympanum located just anterior to the inguinal region.

Key words. - Laos, new species, Philautus, Rhacophoridae

Figure 1A. Map showing the type locality (black dot) of Philautus petilus sp. nov. in Phongsaly Province, northern Laos.

Figure 1A-C. A. Map showing the type locality (black dot) of Philautus petilus sp. nov. in Phongsaly Province, northern Laos. B. The adult female holotype (FMNH 257902) of Philautus petilus sp. nov., anesthetized prior to preservation. Photograph by Bryan L. Stuart. C. The hand of the adult female holotype (FMNH 257902) of Philautus petilus sp. nov. in preservation. Photograph by Nikolai L. Orlov.

Philautus petilus Stuart & Heatwole, 2004

Type locality: Phou Dendin National Biodiversity Conservation Area, Phongsaly District, Phongsaly Province, Laos, at 600 m elevation.

Bryan L. Stuart and Harold Heatwole. 2004. A New Philautus (Amphibia: Rhacophoridae) from northern Laos. Asiatic Herpetological Research. 10: 17-21. http://asiatic-herpetological.org/Archive/Volume%2010/10_04.pdf

 asiatic-herpetological.org/Archive.htm
 researchgate.net/publication/228506727_A_new_Philautus_from_northern_Laos